Anti-Robo1 (extracellular) Antibody | Alomone Labs ANR-181 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Robo1 (extracellular) Antibody

catalog :

ANR-181

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1, 2. Anti-Robo1 (extracellular) Antibody (#ANR-181), (1:200). 3, 4. Anti-Robo1 (extracellular) Antibody, preincubated with Robo1 (extracellular) Blocking Peptide (#BLP-NR181) (#BLP-NR181).

image 2 :

Expression of Robo1 in mouse cortex - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Robo1 (extracellular) Antibody (#ANR-181), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Robo1 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with Robo1 (extracellular) Blocking Peptide (#BLP-NR181), suppresses staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-181

SKU :

ANR-181-CF_0.2 ml

Product Name :

Anti-Robo1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Y6N7

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR181

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DGVLVSTQDSRIKQLE, corresponding to amino acid residues 491 - 506 of human Robo1

Product Page - Scientific background :

Roundabout (Robo) receptors are a conserved family of four single pass transmembrane proteins1. All members have multiple immunoglobulin-like domains in the extracellular N-terminal region, three extracellular fibronectin type II domains, and cytoplasmic conserved domains. Robos also undergo preoteolytic processing, important for their signaling process2. They serve as receptors for Slit proteins, where upon activation lead to pleiotropic effects including axonal guidance1, axonal repulsion3,4, and neural migration5.Slit/Robo signaling mainly prevents axons from migrating to inappropriate locations during the assembly of the nervous system6.Robos are mainly expressed in the nervous system, but are also detected in vascular endothelial and muscle cells7. In non-neuronal tissues, Slit/Robo proteins are key oncogenes and tumor suppressors in various types of cancers. Robo1 expression is elevated in breast cancer and colorectal cancer, and has an overall oncogenic role1.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1

Short Description :

A Rabbit Polyclonal Antibody to Robo1

Long Description :

Anti-Robo1 (extracellular) Antibody (#ANR-181) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize Robo1 from human, rat, and mouse samples.

Negative Control :

BLP-NR181

Positive Control :

Synonyms :

Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ROBO1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage