Anti-Robo1 (extracellular)-FITC Antibody | Alomone Labs ANR-181-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Robo1 (extracellular)-FITC Antibody

catalog :

ANR-181-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of Robo1 by direct flow cytometry in live intact mouse J774 macrophage cell line:

image 2 :

Cell surface detection of Robo1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:

product information

CAT :

ANR-181-F

SKU :

ANR-181-F_15 mcl

Product Name :

Anti-Robo1 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Y6N7

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR181

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DGVLVSTQDSRIKQLE, corresponding to amino acid residues 491 - 506 of human Robo1

Product Page - Scientific background :

Roundabout (Robo) receptors are a conserved family of four single pass transmembrane proteins1. All members have multiple immunoglobulin-like domains in the extracellular N-terminal region, three extracellular fibronectin type II domains, and cytoplasmic conserved domains. Robos also undergo preoteolytic processing, important for their signaling process2. They serve as receptors for Slit proteins, where upon activation lead to pleiotropic effects including axonal guidance1, axonal repulsion3,4, and neural migration5.Slit/Robo signaling mainly prevents axons from migrating to inappropriate locations during the assembly of the nervous system6.Robos are mainly expressed in the nervous system, but are also detected in vascular endothelial and muscle cells7. In non-neuronal tissues, Slit/Robo proteins are key oncogenes and tumor suppressors in various types of cancers. Robo1 expression is elevated in breast cancer and colorectal cancer, and has an overall oncogenic role1.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1

Short Description :

A Rabbit Polyclonal Antibody to Robo1 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-Robo1 (extracellular) Antibody (#ANR-181) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize Robo1 from mouse, rat and human samples. Anti-Robo1 (extracellular)-FITC Antibody (#ANR-181-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ROBO1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANR-181), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage