Anti-Progranulin Antibody | Alomone Labs ANR-178 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Progranulin Antibody

catalog :

ANR-178

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and rat spleen membranes (lanes 2 and 4): - 1-2. Anti-Progranulin Antibody (#ANR-178), (1:400). 3-4. Anti-Progranulin Antibody, preincubated with Progranulin Blocking Peptide (BLP-NR178).

image 2 :

Expression of progranulin in rat hypothalamus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Progranulin Antibody (#ANR-178), (1:100), followed by goat anti-rabbit-AlexaFluor-488. A. Progranulin immunoreactivity (green) appears in neurons (arrows pointing to the left) and along the wall of 3rd ventricle (arrows pointing to the right). B. Pre-incubation of the antibody with Progranulin Blocking Peptide (BLP-NR178), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V= Third ventricle.

product information

CAT :

ANR-178

SKU :

ANR-178-CF_0.2 ml

Product Name :

Anti-Progranulin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P28798

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR178

Homology :

Mouse - 15 out of 16 amino acid identical; Human- 14 out of 16 amino acid identical; Rat- 13 out of 16 amino acid identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Secreted

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CDLIQSK(S)LSKNYTTD, corresponding to amino acid residues 252 - 267 of mouse GRN

Product Page - Scientific background :

The Progranulin (PGRN) protein is encoded by the GRN gene composed of 13 exons, although exon 1 is non-coding. PGRN is a secreted glycoprotein with growth factor like properties, consisting in 7.5 tandem repeats of a 12-cysteine module called granulin or epithelin domain separated by linker regions. The 12-cysteine motif forms six disulfide bridges with the peptide backbone adopting a unique three-dimensional structure of a parallel stack of beta hairpin1,2.PGRN is synthesized as a 68.5 kDa protein that is N-glycosylated to become a mature secreted 88 kDa protein3. Secreted PGRN is cleaved into 6 kDa granulin peptides, each composed of cysteine repeat motifs. Cleavage of PGRN occurs at the intra-linker spacer regions by several proteases including elastase4, proteinase5, matrix metalloproteinase-146 and ADAM metallopeptidase with thrombospondin type 1 motif7. PGRN cleavage is inhibited by secretory leukocyte protease inhibitor (SLPI)4 and high-density lipoprotein (HDL)/Apolipoprotein A-I complex8.PGRN is expressed in several tissues showing a prominent expression in epithelial, immune and, neuronal cells9. In particular, a strong expression of PGRN mRNA was found in highly proliferative epithelia including those of the intestinal crypt, skin, and reproductive tracts9. In contrast, PGRN shows low expression in less proliferative and highly differentiated cells such as those of alveolar lung and renal tube epithelia9.PGRN shows high expression in peripheral immune system tissues such as spleen and lymph nodes9 and is also highly expressed in the central nervous system (CNS) with expression in both neurons and neuroinflammatory cells. PGRN has been detected in neurons of the neocortex, hippocampus, arcuate nucleus and ventromedial hypothalamus, Purkinje cells of the cerebellum and in motor neurons9. In the CNS, PGRN can function as neurotrophic growth factor increasing cell viability10 and neurite outgrowth [11]. PGRN knockdown in rat hippocampal neurons results in decreased neuronal arborization and length and synaptic densities12 showing its role in neuronal morphology and establishing interneuron connections.Several proteins have been identified as receptors for GPRN, including Sortilin, which is considered the major regulator of GPRN in the brain, as well as, EphA2, LRP1, Notch and more13.Loss of function mutations in the GRN gene, cause a range of neurodegenerative disorders including frontotemporal dementia (FTD), amyotrophic lateral sclerosis (ALS), Alzheimer's and Parkinson's disease 13.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Granulin Precursor, PGRN, Epithelin Precursor, Glycoprotein 88, GP88, PC Cell-Derived Growth Factor, PCDGF, GRN

Short Description :

A Rabbit Polyclonal antibody to Progranulin

Long Description :

Anti-Progranulin Antibody (#ANR-178) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Progranulin from rat, mouse and human samples.

Negative Control :

BLP-NR178

Positive Control :

Synonyms :

Granulin Precursor, PGRN, Epithelin Precursor, Glycoprotein 88, GP88, PC Cell-Derived Growth Factor, PCDGF, GRN

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRN

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage