antibody

Anti-RAGE (extracellular) Antibody

ANR-176

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

ANR-176-CF_0.2 ml

Anti-RAGE (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q15109

IFC WB

Human Rat Mouse

Rabbit

BLP-NR176

Mouse, rat - 17/18 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

(C)GKETKSNYRVRVYQIPGK, corresponding to amino acid residues 106 - 123 of human RAGE

Receptor for advanced glycation end-products (RAGE) is a cell surface transmembrane multi-ligand receptor belonging to the immunoglobulin superfamily, encoded by the AGER gene.The link between the RAGE and its multiple ligands, including Glycation End Product (AGEs) triggers a cascade of intracellular events and transcription of genes, as well as other reactions such as the formation of oxidative stress. These reactions are involved in neurological and vascular complications of diabetes, Alzheimer's Disease, arthritis, and some cancers. In addition, chronic stimulation of RAGE triggers different signaling pathways and reactions1,2.RAGE is a 35 kDa protein mainly expressed in the lungs and exists under two main forms: membrane-bound RAGE (mRAGE) and soluble RAGE (sRAGE). Membrane-bound RAGE has three domains: an extracellular domain that recognizes and binds RAGE ligands, a hydrophobic transmembrane domain, and a charged cytoplasmic domain that functions in intracellular signaling. Soluble RAGE contains only the extracellular domain and is a product of either alternative splicing events or the proteolytic cleavage of mRAGE. Because it can bind ligands but cannot transduce signals intracellularly, sRAGE is a decoy receptor that sequesters RAGE ligands and prevents inflammatory responses3.Use of sRAGE, RAGE blocking antibody, or a small molecule inhibitors to interfere with RAGE signaling may become novel therapeutic approaches to inhibit RAGE-mediated disease4.

IFC WB

Alomone Labs

Advanced Glycosylation End Product-Specific Receptor, AGER, Receptor For Advanced Glycosylation End Products, SCARJ1

A Rabbit Polyclonal Antibody to RAGE

Anti-RAGE (extracellular) Antibody (#ANR-176) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize RAGE from human, rat, and mouse samples. This antibody will recognize both the membrane-bound and the soluble form of the RAGE receptor.

BLP-NR176

NA

Advanced Glycosylation End Product-Specific Receptor, AGER, Receptor For Advanced Glycosylation End Products, SCARJ1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

AGER

This antibody will recognize both the membrane-bound and the soluble form of the RAGE receptor.

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:600