antibody

Anti-TMEM119 (extracellular)-PE Antibody

ANR-175-PE

polyclonal

domestic rabbit

nonconjugated

mouse, rat

ANR-175-PE_15 mcl

Anti-TMEM119 (extracellular)-PE Antibody

Antibodies

Antibodies

Polyclonal

Q8R138

FC LCI

Rat Mouse

Rabbit

BLP-NR175

Rat – 12 out of 15 amino acid residues identical. This antibody will not recognize human TMEM119

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

1% BSA, 0.05% NaN3

Extracellular, N-term.

R-Phycoerythrin (R-PE)

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119

Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1. Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2. Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytes/macrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

FC LCI

Alomone Labs

Transmembrane Protein 119, Osteoblast induction factor, OBIF

A Rabbit Polyclonal Antibody to TMEM119 conjugatd to R-Phycoerythrin (R-PE) fluorophore

Anti-TMEM119 (extracellular) Antibody (#ANR-175) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples. Anti-TMEM119 (extracellular)-PE Antibody (#ANR-175-PE) to R-Phycoerythrin (R-PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

RIC-001-PE

Transmembrane Protein 119, Osteoblast induction factor, OBIF

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

Affinity purified on immobilized antigen.

Rabbit IgG Isotype Control-PE

41116161

Western blot analysis (unlabeled antibody, #ANR-175), and direct flow cytometry (labeled antibody).