Anti-TMEM119 (extracellular)-APC Antibody | Alomone Labs ANR-175-APC product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TMEM119 (extracellular)-APC Antibody

catalog :

ANR-175-APC

clonality :

polyclonal

host :

domestic rabbit

conjugate :

APC

clone name :

reactivity :

mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of TMEM119 by direct flow cytometry in live intact mouse BV-2 microglia cells: Cell surface detection of TMEM119 by direct flow cytometry in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-APC. Green line: Cells + Anti-TMEM119 (extracellular)-APC Antibody (#ANR-175-APC), (2.5µg) .

product information

CAT :

ANR-175-APC

SKU :

ANR-175-APC_50 mcl

Product Name :

Anti-TMEM119 (extracellular)-APC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8R138

Applications :

FC LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR175

Homology :

Rat - 12 out of 15 amino acid residues identical This antibody will not recognize human TMEM119

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus.

Label :

Allophycocyanin (APC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119

Product Page - Scientific background :

Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytes/macrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Transmembrane Protein 119, Osteoblast induction factor, OBIF

Short Description :

A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)

Long Description :

Anti-TMEM119 (extracellular) Antibody (#ANR-175) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples. Anti-TMEM119 (extracellular)-APC Antibody (#ANR-175-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-APC

Positive Control :

Synonyms :

Transmembrane Protein 119, Osteoblast induction factor, OBIF

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TMEM119

AB Specifictiy :

This antibody will not recognize human TMEM119.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANR-175), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage