antibody

Anti-Contactin-2/CNTN2 (extracellular) Antibody

ANR-172

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

ANR-172-CF_0.2 ml

Anti-Contactin-2/CNTN2 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q61330

IHC WB

Human Rat Mouse

Rabbit

BLP-NR172

Rat, human - 14 out of 15 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)KMNGTEMNLEPGSRH, corresponding to amino acid residues 76 - 90 of mouse CNTN2

Contactin-2 (also known as Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1, CNTN2) is a cell adhesion molecule that belongs to the immunoglobulin superfamily (IgSF).IgSF proteins mediate protein-protein interactions. They are either secreted or localized to the cell surface. Many of the cell surface IgSF proteins are adhesion molecules (IgCAMs) that mediate interactions among neurons, and between neurons and glia.1The immunoglobulin superfamily is divided into subgroups, one of which includes the contactin subfamily that consists of six members (Contactin-1 to Contactin-6). All six molecules of the contactin subgroup have six extracellular immunoglobulin-like domains and four fibronectin III-like domains, are anchored to the lipid bilayer of the cell membrane via glycosylphosphatidylinositol (GPI), and are found on the membrane surface.2 Contactin-2 is primarily expressed on the axonal and synaptic membranes. It is expressed in hippocampal pyramidal cells, cerebellar granule cells, the juxtaparanodal regions of myelinated nerve fibers, and frontal and temporal lobes. Contactin-2 is a multifunctional protein; in the embryonic nervous system, it plays important roles in axonal elongation, axonal guidance, and cellular migration. In the postnatal nervous system, it plays a role in neuronal fasciculation, axonal domain organization, formation of myelinated nerve fibers, and neuron-glia interaction.3Contactin-2 interacts with contactin associated protein-like 2 (caspr2) at the juxtaparanodal region of nodes of Ranvier. This association is necessary for the maintenance of voltage-gated potassium channels (VGKCs) at juxtaparanodal regions of myelinated axons.Caspr2 autoantibodies disrupt the binding of Caspr2 to contactin-2, resulting in hyperexcitability of peripheral nerves. These autoantibodies have been associated with a form of acquired peripheral nerve hyperexcitability (acquired neuromyotonia) and autoimmune encephalitis. 4Contactin-2 is also a target protein of autoantibodies in patients with Multiple Sclerosis (MS)5. It was identified as one of the elevated cerebrospinal fluid proteins in a proteomics study in pediatric MS patients.6Contactin-2 interacts with proteins involved in alzheimer's disease (AD) pathogenesis, such as amyloid precursor protein (APP) and beta-secretase 1 (BACE1). These interactions may influence the production of Aβ peptide and the subsequent formation of amyloid plaques. Single nucleotide polymorphisms (SNPs) in the gene encoding contactin-2 (CNTN2) are associated with AD. In addition, higher levels of contactin-2 have been reported in AD CSF pools using proteomics approaches.3In addition, Contactin-2 is reported to be overexpressed in malignant gliomas, where it is involved in the growth and differentiation of glioma cells. It is also associated with development of oligodendrogliomas.2

IHC WB

Alomone Labs

Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1

A Rabbit Polyclonal Antibody to Contactin-2 (extracellular)

Anti-Contactin-2/CNTN2 (extracellular) Antibody (#ANR-172) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Contactin-2 from rat, mouse and human samples.

BLP-NR172

NA

Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

CNTN2

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:200

1:200