Anti-NGL-3/LRRC4B (extracellular) Antibody | Alomone Labs ANR-163 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NGL-3/LRRC4B (extracellular) Antibody

catalog :

ANR-163

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat new born brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 1,2. Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200).3,4. Anti-NGL-3/LRRC4B (extracellular) Antibody, preincubated with NGL-3/LRRC4B (extracellular) Blocking Peptide (#BLP-NR163).

image 2 :

Expression of NGL-3 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NGL-3 immunoreactivity (green) is detected in the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-163

SKU :

ANR-163-CF_0.2 ml

Product Name :

Anti-NGL-3/LRRC4B (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P0C192

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR163

Homology :

Rat - identical; human - 13 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)PGEEAQQPRGTEKE, corresponding to amino acid residues 498-511 of mouse NGL-3

Product Page - Scientific background :

NGL-3 (Netrin G ligand 3) is a postsynaptic cell adhesion molecule that is a member of the NGL family. NGLs are proteins involved in the regulation of various steps in synapse formation. Members of this family share a common domain structure that contains: nine Leucine rich repeats (LRRs), an immunoglobulin (Ig) domain in the extracellular region, a single trans membrane domain and a cytoplasmic region that ends with a PDZ domain-binding motif. This motif mediates the interaction with PSD-95, an abundant postsynaptic scaffolding protein1,2.The NGL family is comprised of three proteins: NGL-1, 2, and 3. NGL-1 and NGL-2 interact through their extracellular domain with the GPI anchored membrane proteins Netrin G1 and G2 respectively2-4. This interaction is important for regulation of structural and functional excitatory synapse development4.NGL-3 trans-synaptically interacts with the leukocyte common antigen-related (LAR) protein which is well-known for its involvement in axon guidance and presynaptic differentiation. This interaction induces pre- and postsynaptic differentiation in contacting axons and dendrites, respectively, and regulates excitatory synapse formation2,5.Mice lacking NGL-3 (Ngl3−/−) show markedly suppressed normal brain development and postnatal survival and growth6. NGL-3 overexpression and knockdown in cultured neurons result in bidirectional changes in the number of excitatory synapses and spontaneous excitatory synaptic transmission5.NGL-3 mRNA and proteins are mainly detected in synaptic fractions of the brain and levels gradually increase during the first 3 weeks of postnatal rat brain development6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4

Short Description :

A Rabbit Polyclonal Antibody to NGL-3/LRRC4B

Long Description :

Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize LRRC4B from rat, mouse, and human samples.

Negative Control :

BLP-NR163

Positive Control :

Synonyms :

Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LRRC4B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage