Anti-NGL-2/LRRC4 (extracellular) Antibody | Alomone Labs ANR-162 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NGL-2/LRRC4 (extracellular) Antibody

catalog :

ANR-162

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain synaptosome preparation (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200). 3-4. Anti-NGL-2/LRRC4 (extracellular) Antibody, preincubated with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162).

image 2 :

Expression of NGL-2/LRRC4 in rat hypothalamus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. NGL-2/LRRC4 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third cerebral ventricle

product information

CAT :

ANR-162

SKU :

ANR-162-CF_0.2 ml

Product Name :

Anti-NGL-2/LRRC4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q99PH1

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR162

Homology :

Rat - identical, Human 13 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)STDTTDKMQTSLDE, corresponding to amino acid residues 507 - 520 of mouse LRRC4

Product Page - Scientific background :

The leucine-rich repeat (LRR) superfamily is a diverse protein family that consist of leucine-rich domains (LRR domains). The LRR domain is known to regulate protein-protein interaction or cell adhesion. Large portion of LRR proteins mediate the differentiation and development of nervous tissues1. One member of the LRR superfamily is leucine rich repeat containing protein 4 (LRRC4), also known as netrin-G ligand-2 (NGL-2), and serves as a receptor for netrin-G2 (NTNG2). In addition to the LRR domain LRRC4 consist of immunoglobulin (Ig) domain in the extracellular region, a transmembrane domain, and a cytoplasmic region that terminates in a protein-protein interaction domain (PDZ)-binding motif. LRRC4 regulates the formation of excitatory synapses and promotes axon differentiation2.Functionally, deletion of LRRC4 in mice was found to suppress excitatory synapse development and synaptic transmission. In addition, LRRC4 gene has been identified as tumor suppressor3. In human it has been implicated in autism spectrum disorder (ASD), schizophrenia, and intellectual disability. Moreover, netrin-G2, the presynaptic ligand of LRRC4, is linked to schizophrenia and bipolar disorder4-5.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Netrin-G2 Ligand, Brain Tumor-Associated Protein BAG, Leucine-Rich Repeat-Containing Protein 4

Short Description :

A Rabbit Polyclonal antibody to NGL-2/LRRC4 (extracellular)

Long Description :

Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize LRRC4 from rat, mouse and human samples.

Negative Control :

BLP-NR162

Positive Control :

Synonyms :

Netrin-G2 Ligand, Brain Tumor-Associated Protein BAG, Leucine-Rich Repeat-Containing Protein 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LRRC4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage