Anti-ADAM10 (extracellular) Antibody | Alomone Labs ANR-156 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ADAM10 (extracellular) Antibody

catalog :

ANR-156

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-ADAM10 (extracellular) Antibody (#ANR-156), (1:500). 3-4. Anti-ADAM10 (extracellular) Antibody, preincubated with ADAM10 (extracellular) Blocking Peptide (BLP-NR156).

image 2 :

Expression of ADAM10 in mouse parietal cortex. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ADAM10 (extracellular) Antibody (#ANR-156), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. ADAM10 immunoreactivity (green) appears in neuronal profiles (horizontal arrows). B. Pre-incubation of the antibody with ADAM10 (extracellular) Blocking Peptide (BLP-NR156), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-156

SKU :

ANR-156-CF_0.2 ml

Product Name :

Anti-ADAM10 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35598

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR156

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CTPGESKNLGQKENG, corresponding to amino acid residues 400 - 414 of mouse ADAM10

Product Page - Scientific background :

Disintegrin and metalloproteinase domain-containing protein 10, also known as ADAM10, is a membrane-anchored protease that belongs to the ADAM protease family. ADAMs are type I transmembrane proteins with a large extracellular domain and a shorter cytosolic part, they contain a metalloprotease domain, a disintegrin domain, a cystein-rich domain, an EGF-like domain, a transmembrane domain and a cytoplasmic tail2. 37 murine and 22 human ADAMs are known and only some of them contain the catalytically relevant domain (HEXGHXXGXXHD) and are therefore able to cleave the extracellular domains of several membrane-bound proteins in a process known as 'ectodomain shedding'1. This process represents a unique and irreversible posttranslational event regulating the function and half-life of many intracellular and extracellular proteins and hence the ADAM family protein's activity is essential for regulation of various cellular functions2. ADAM10 has many substrates, among which are cellular adhesion molecules such as cadherin, receptors and ligands3. it is also capable of anti-amyloidogenic proteolysis of the amyloid precursor protein, this is why ADAM10 mutations were shown to be correlated with Alzheimer's disease2. In addition, it was shown to involved in various human diseases and disorders such as Huntington's disease, psoriasis, lupus, and multiple types of cancer. The central role of ADAM10 in various cell processes and diseases reveals the potency of ADAM10-targeting drug development as a future therapeutic option2.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Disintegrin And Metalloproteinase Domain-Containing Protein 10, ADAM Metallopeptidase Domain 10, CD156c

Short Description :

A Rabbit Polyclonal Antibody to ADAM10 (extracellular)

Long Description :

Anti-ADAM10 (extracellular) Antibody (#ANR-156) is a highly specific antibody directed against an extracellular epitope of mouse ADAM10. The antibody can be used in western blot, Indirect flow cytometry and immunohistochemistry. It has been designed to recognize ADAM10 from mouse, rat and human samples.

Negative Control :

BLP-NR156

Positive Control :

Synonyms :

Disintegrin And Metalloproteinase Domain-Containing Protein 10, ADAM Metallopeptidase Domain 10, CD156c

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADAM10

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage