antibody

Anti-ADAM23 (extracellular) Antibody

ANR-155

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

ANR-155-CF_0.2 ml

Anti-ADAM23 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q9R1V7

IFC IHC LCI WB

Human Rat Mouse

Rabbit

BLP-NR155

Human, Rat - 14 out of 15 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)HDFSKYRQRIKQHAD, corresponding to amino acid residues 367 - 381 of mouse ADAM23

A Disintegrin And Metalloprotease 23 (ADAM23) is a member of the ADAMs family of transmembrane proteins, mostly expressed in the nervous system, and involved in traffic and stabilization of Kv1-potassium channels, synaptic transmission, neurite outgrowth, neuronal morphology and cell adhesion. ADAM23 has been linked to human pathological conditions, such as epilepsy, cancer metastasis and cardiomyopathy.[1]The ADAMs are a family of transmembrane and secreted proteins of approximately 750 amino acids in length. They differ in structural organization, function and localization. Their domains, catalytic or non-catalytic, determine their substrate recognition and protease activity. Some ADAMs show sheddase activity, which modulates functional proteins such as TNFs, growth factors, cytokines, and adhesion proteins. [2]ADAM23 is a transmembrane type I glycoprotein. Since its metalloprotease domain is inactive, it appears to function mainly through the disintegrin domain. ADAM23 has been shown to regulate mechanisms in epilepsy, inflammation and tumor establishment through interaction with different members of integrin receptors.[3]ADAM23 is thought to form a trans-synaptic complex with ADAM22 and LGI1. ADAM23 binds Kv1.1/Kv1.4 potassium channels, ADAM22 binds post-synaptic AMPA receptors, and LGI1 align these pre- and post-synaptic elements. The interaction of LGI1 and the ADAM proteins appears to regulate excitability of CNS neurons. In addition to binding to LGI1, ADAM23 also regulates the amount of LGI1 molecules at the axon initial segment, by modulating the trafficking, export and expression of LGI1.[4] In knockout studies in mice, aberrant LGI1 regulation of neuronal morphology through ADAM23 protein resulted in Autosomal dominant lateral temporal epilepsy (ADLTE). Autoantibodies directed at LGI1 have been shown to disrupt the trans-synaptic complex and are associated with autoimmune encephalitis. ADAM23-null mice had aberrant dendrite morphology and exhibited seizures. Reduced ADAM23 expression lowered seizure threshold.[5]ADAM23 has a specific interaction with alpha(v)beta(3) integrin mediated by the disintegrin domain. ADAM23 knockdown cells showed enhanced migration and adhesion to alpha(v)beta(3) integrin ligands. Expression of this integrin in the activated form has been shown to promote metastasis formation. This suggests a functional role of ADAM23 during metastatic progression by negatively modulating alpha(v)beta(3) integrin activation. It has been shown that the ADAM23 gene is frequently silenced in different types of tumors, including melanoma, glioma, prostate, ovary, and breast cancer.[6]

IFC IHC LCI WB

Alomone Labs

Disintegrin and Metalloproteinase Domain-Containing Protein 23, MDC3

A Rabbit Polyclonal antibody to ADAM23 (extracellular)

Anti-ADAM23 (extracellular) Antibody (#ANR-155) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize ADAM23 from mouse, rat and human samples.

BLP-NR155

NA

Disintegrin and Metalloproteinase Domain-Containing Protein 23, MDC3

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ADAM23

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:300

1:200