Anti-ASTN1 (extracellular) Antibody | Alomone Labs ANR-151 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ASTN1 (extracellular) Antibody

catalog :

ANR-151

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Olingy C, San Emeterio C, Ogle M, Krieger J, Bruce A, Pfau D, et al. Non-classical monocytes are biased progenitors of wound healing macrophages during soft tissue injury. Sci Rep. 2017;7:447 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 1,2. Anti-ASTN1 (extracellular) Antibody (#ANR-151), (1:200).3,4. Anti-ASTN1 (extracellular) Antibody, preincubated with ASTN1 (extracellular) Blocking Peptide (#BLP-NR151).

image 2 :

Expression of Astrotactin-1 in mouse hippocampus and cortex - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASTN1 (extracellular) Antibody (#ANR-151), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Astrotactin-1 immunoreactivity (green) is observed in neurons (arrows) of hippocampal CA3 pyramidal layer (P). B. Astrotactin-1 staining (green) appears in neurons of cortex (arrows).

product information

CAT :

ANR-151

SKU :

ANR-151-CF_0.2 ml

Product Name :

Anti-ASTN1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61137

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR151

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RQEKVTDRMDHSK, corresponding to amino acid residues 1068-1080 of mouse Astrotactin-1

Product Page - Scientific background :

Astrotactin 1 (ASTN-1) is an integral membrane perforin-like protein known to play a critical role in neurodevelopment. The protein is responsible for the formation of neuron-glial fiber contacts in the cerebellum where it plays an important role in neuronal migration1,2.Astrotactin 1 is also expressed in post-mitotic neuronal precursors of the cerebellum, hippocampus, cerebrum and olfactory bulb. The protein demonstrates an active role in the establishment of laminar structures1.Astrotactin 1 structure reveals a large extracellular C-terminal domain that is complemented by an N-terminal cytosolic domain separated by two transmembrane α-helices1.Studies have found that genetic variations associated with Astrotactin 1 are connected to various neurodevelopmental disorders and other neurological pathologies including Alzheimer's disease1. In addition, ASTN1 null mice exhibit slow migration rates of cerebellar granule cells, smaller cerebellar size and reduced glial neuron binding2 and poorer balance and coordination than wild-type1. Conversely, increased levels of Astrotactin 1 are detected in rat brain following hippocampal injury, linking the protein to repair processes.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Astrotactin-1

Short Description :

A Rabbit Polyclonal Antibody to Astrotactin-1

Long Description :

Anti-ASTN1 (extracellular) Antibody (#ANR-151) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Astrotactin-1 from rat, mouse, and human samples.

Negative Control :

BLP-NR151

Positive Control :

Synonyms :

Astrotactin-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ASTN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage