Anti-LRRTM2 (extracellular) Antibody | Alomone Labs ANR-142 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LRRTM2 (extracellular) Antibody

catalog :

ANR-142

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-LRRTM2 (extracellular) Antibody (#ANR-142), (1:200). 2. Anti-LRRTM2 (extracellular) Antibody, preincubated with LRRTM2 (extracellular) Blocking Peptide (BLP-NR142).

image 2 :

Expression of LRRTM2 in mouse parietal cortex. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-LRRTM2 (extracellular) Antibody (#ANR-142), (1:400), followed by goat anti-rabbit-AlexaFluor-488. A. LRRTM2 immunoreactivity (green) appears in cortical neurons in layer 5 (arrows). B. Pre-incubation of the antibody with LRRTM2 (extracellular) Blocking Peptide (BLP-NR142), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-142

SKU :

ANR-142-CF_0.2 ml

Product Name :

Anti-LRRTM2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BGA3

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR142

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CDSQGFHSVPNATDK, corresponding to amino acid residues 47 - 61 of mouse LRRTM2

Product Page - Scientific background :

Leucine-rich repeat transmembrane neuronal proteins (LRRTMs) function as postsynaptic organizers that induce excitatory synapses. There are four related LRRTM genes in mammals, and all are enriched in brain and expressed from postnatal day one or earlier1. LRRTM2 is the most synaptogenic isoform of the family, and its expression is strongly restricted to excitatory synapses in mature neurons. It consists of leucine-rich repeat (LRR) domain in the N-terminal extracellular domain, followed by a single transmembrane domain and a cytoplasmic region contains a PDZ domain binding motif2. LRRTM2 can induce clustering of NMDA receptors, PSD-95 and SynGAP in postsynaptic membrane. PSD-95 function as a scaffold to cluster these postsynaptic proteins. It was found that LRRTM2 deletion in mice leading to dramatically impairs basal synaptic transmission and disrupts long-term potentiation, a prominent form of synaptic plasticity that is critical for learning and memory3. LRRTM2 responsible for the structural linkage mediating nanocolumn alignment of AMPARs, a protein critical for the strength of basal synaptic transmission3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Leucine-rich repeat transmembrane neuronal protein 2, Leucine-Rich Repeat Neuronal 2 Protein, LRRN2

Short Description :

A Rabbit Polyclonal antibody to LRRTM2 (extracellular)

Long Description :

Anti-LRRTM2 (extracellular) Antibody (#ANR-142) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize LRRTM2 from rat, mouse and human samples.

Negative Control :

BLP-NR142

Positive Control :

Synonyms :

Leucine-rich repeat transmembrane neuronal protein 2, Leucine-Rich Repeat Neuronal 2 Protein, LRRN2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LRRTM2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage