Anti-SARM1 Antibody | Alomone Labs ANR-138 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SARM1 Antibody

catalog :

ANR-138

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of newborn rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):

image 2 :

Western blot analysis of human SHSY-5Y neuroblastoma cell line lysate (lanes 1 and 4), h uman A RPE-19 retinal epithelial cell line lysate (lanes 2 and 5) and h uman L NCa P prostate carcinoma cell line lysate (lanes 3 and 6):

image 3 :

Expression of SARM1 in rat hippocampus

product information

CAT :

ANR-138

SKU :

ANR-138-CF_0.2 ml

Product Name :

Anti-SARM1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6PDS3

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR138

Homology :

Rat, human – identical.

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular (ARM 8 repeat).

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KTKVFSDIGAIQSLKR, corresponding to amino acid residues 361 - 376 of mouse SARM1

Product Page - Scientific background :

The sterile alpha and TIR motif-containing protein 1 (SARM1) belongs to the family of Toll/interleukin-1 receptor (TIR)-domain-containing proteins1. This protein is characterized by its multidomain structure, including N-terminal Armadillo repeats (ARM), tandem sterile alpha motif (SAM) domains, and a C-terminal catalytic TIR domain2. SARM1 is an intracellular protein predominantly localized in the axons of neurons. It plays a critical role in axonal self-destruction through its NAD+ glycohydrolase activity, which is initiated under pathological conditions such as axonal injury2,3. SARM1 expression is prominent in neuronal populations, particularly within the central and peripheral nervous systems. It is upregulated under stress conditions, such as hypoxia and neuroinflammatory states3. The activation of SARM1 is regulated by nicotinamide mononucleotide (NMN), which disrupts the interaction between the ARM and TIR domains, leading to the formation of TIR-domain assemblies that execute NAD+ cleavage2,4. Biologically, SARM1 is implicated in Wallerian degeneration, a process of axonal degeneration triggered by trauma or neuropathies. It also mediates axonal degeneration in metabolic and toxic neuropathies, such as those induced by high-fat diets or chemotherapy agents like paclitaxel3,5. In vitro studies have shown that genetic deletion of SARM1 protects neurons from degeneration by maintaining NAD+ levels. Similarly, in vivo studies reveal its role in preserving axonal integrity in models of traumatic, toxic, and metabolic insults3,5. Dysregulation of SARM1 activity is associated with various neurodegenerative conditions, including peripheral neuropathies, glaucoma, and traumatic brain injuries2,3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Sterile Alpha And TIR Motif-Containing Protein 1, NAD(+) Hydrolase SARM1, Sterile Alpha And Armadillo Repeat Protein

Short Description :

A Rabbit Polyclonal Antibody to SARM1

Long Description :

Anti-SARM1 Antibody (#ANR-138) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SARM1 from mouse, rat and human samples.

Negative Control :

BLP-NR138

Positive Control :

Synonyms :

Sterile Alpha And TIR Motif-Containing Protein 1, NAD(+) Hydrolase SARM1, Sterile Alpha And Armadillo Repeat Protein

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SARM1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Standard quality control of each lot :

Western blot analysis.

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage