antibody

Anti-SORCS2 (extracellular) Antibody

ANR-135

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

western blot, immunohistochemistry

ANR-135-CF_0.2 ml

Anti-SORCS2 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q9EPR5

IHC WB

Rat Mouse

Rabbit

BLP-NR135

Rat - identical, Human - 12 out of 13 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-term.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)DYLRPPSTDMNGK, corresponding to amino acid residues 478 - 490 of mouse SORCS2

SORCS2 is a member of the Vps10p-domain receptor family, which comprises five multifunctional neuronal receptors, type I transmembrane receptors Sortilin in humans or sortilin in mice (encoded by the gene SORT1 or Sort1, respectively), SORLA/SorLA (encoded by SORL1/Sorl1) and SORCS1-3/SorCS1-3 (encoded by SORCS1- 3/Sorcs1-3)1, 2. The receptor family is expressed predominantly in the nervous system (both developing and adult) and involved in intracellular sorting and trafficking of various neurotrophic factors and their precursor forms, as well transmembrane receptors and synaptic proteins1.This gene family has been implicated in a broad range of cellular processes, including neuronal health, differentiation and synaptic plasticity1, 2. The SorCS subfamily contains three closely related members, with at least 49% identity between the VPS10 domains3. SorCS members also contain a leucine rich region (LRR), with 63% identity between SorCS1 and SorCS3 versus only 35% identity between SorCS1 and SorCS23. The three SorCS genes are differentially expressed and regulated in mature neurons3.Since their discovery a growing number of studies have linked SorCS genes to diverse neurodegenerative diseases such as autism, schizophrenia, bipolar disorder, attention deficit hyperactivity disorder, late-onset Alzheimer disease and multiple sclerosis4-6. SorCS2 is the VPS10 family member that is the least characterized. Ten years after its identification7, a first role for SorCS2 in proneurotrophin-mediated neurodegeneration as co-receptor of the p75NTR was uncovered8. Roles for SorCS2 in amyotrophic lateral sclerosis, bipolar disorder and schizophrenia have been suggested5, 6, 9, but the mechanism of action of SorCS2 is poorly understood10, though recent studies have shown that SorCS2 regulates pro-BDNF dependent synapse plasticity11, 12. Furthermore, SorCS2 has been linked to obesity and colorectal cancer, suggesting a role akin to Sortilin's for SorCS2 in these diseases13-15.SorCS2 is localized predominantly in the brain, but also in heart, liver and kidney7, 16. Unlike Sortilin, SorCS2 is preferentially located at the cell surface in mature neurons of the central nervous system (CNS) and glia of the peripheral nervous system (PNS), making it the preferred co-receptor for p75-dependent apoptosis17. SorCS2 contains a leucine-rich region linking the VPS10 domain and the transmembrane domain. It has been shown to exist both as a single chain receptor in the neurons of the CNS and as a cleaved two-chains receptor in the glia of the PNS18. The proteolytic processing site yielding this two-chain version of SorCS2 is located in the leucine-rich region at residue S1031. SorCS2 interacts with proneurotrophins8, 11, 12. Both single and double-chain SorCS2 interact with proneurotrophins (and with less affinity to mature neurotrophins) and form a ternary complex with p75NTR, but the single chain receptor induces growth cone collapse of dopaminergic neurons while the double-chain receptor transduces apoptosis signals in PNS glia18.

IHC WB

Alomone Labs

Sortilin Related VPS10 Domain Containing Receptor 2, VPS10 Domain-Containing Receptor SorCS2

A Rabbit Polyclonal antibody to SORCS2

Anti-SORCS2 (extracellular) Antibody (#ANR-135) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SORCS2 from rat, mouse and human samples.

BLP-NR135

NA

Sortilin Related VPS10 Domain Containing Receptor 2, VPS10 Domain-Containing Receptor SorCS2

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SORCS2

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:300

1:200