Anti-RGMA (extracellular) Antibody | Alomone Labs ANR-126 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-RGMA (extracellular) Antibody

catalog :

ANR-126

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):

image 2 :

Western blot analysis of human glioblastoma U-87 MG cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4):

image 3 :

Expression of RGMA in mouse hippocampus:

product information

CAT :

ANR-126

SKU :

ANR-126-CF_0.2 ml

Product Name :

Anti-RGMA (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6PCX7

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR126

Homology :

Mouse, human, rat – 16 out of 17 amino acid identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus (pro-domain).

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ERSDSPEI(S)HYEKSFHK, corresponding to amino acid residues 138 - 154 of mouse RGMA

Product Page - Scientific background :

Repulsive Guidance Molecule A (RGMA) is a member of the RGM family, which also includes RGMb (DRAGON) and RGMc (hemojuvelin). It is a glycosylphosphatidylinositol (GPI)-anchored protein characterized by a von Willebrand factor type D domain and a GPI-anchor, facilitating membrane association and soluble forms1,2. This dual functional nature enables RGMA to act both as a localized, membrane-bound signal and as a long-range soluble signaling molecule, expanding its versatility in biological processes3. RGMA plays a critical role in axonal guidance, neuronal differentiation, and survival, primarily by interacting with the receptor neogenin. This interaction also regulates apoptosis via Akt pathway dephosphorylation. RGMA acts as a Bone Morphogenetic Protein (BMP) co-receptor, enhancing Smad-dependent signaling pathways2,3. It is expressed in the central nervous system (CNS), particularly in retinal ganglion cells, midbrain dopaminergic neurons, and the spinal cord, with roles extending from embryonic development to adult neuroplasticity1,3,4. RGMA is implicated in several biological processes, including axonal regeneration and inhibition, neuronal survival, and inflammatory responses. Pathological upregulation of RGMA has been linked to neurodegenerative disorders like multiple sclerosis (MS) and Parkinson’s disease. In MS, RGMA inhibits axonal regeneration, while in Parkinson’s, it contributes to dopaminergic neuron degeneration1,4,5. Research areas benefiting from RGMA immunodetection include neuroregeneration, CNS repair, and therapeutic strategies for neurodegenerative diseases4,5.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Repulsive Guidance Molecule A, RGM Domain Family Member A, Repulsive Guidance Molecule BMP Co-Receptor A, RGMa

Short Description :

A Rabbit polyclonal antibody to RGMA (extracellular).

Long Description :

Anti-RGMA (extracellular) Antibody (#ANR-126) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize RGMA from mouse, rat and human samples.

Negative Control :

BLP-NR126

Positive Control :

Synonyms :

Repulsive Guidance Molecule A, RGM Domain Family Member A, Repulsive Guidance Molecule BMP Co-Receptor A, RGMa

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

RGMA

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage