Anti-Netrin-3 Antibody | Alomone Labs ANR-123 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Netrin-3 Antibody

catalog :

ANR-123

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1-2. Anti-Netrin-3 Antibody (#ANR-123), (1:400). 3-4. Anti-Netrin-3 Antibody, preincubated with Netrin-3 Blocking Peptide (BLP-NR123).

image 2 :

Expression of Netrin-3 in rat spinal cord. - Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Netrin-3 Antibody (#ANR-123), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn region showed Netrin-3 immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with Netrin-3 Blocking Peptide (BLP-NR123), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-123

SKU :

ANR-123-CF_0.2 ml

Product Name :

Anti-Netrin-3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9R1A3

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR123

Homology :

Rat - identical; Human - 13 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Secreted

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CRPARGSYRISLKK, corresponding to amino acid residues 445 - 458 of mouse Netrin-3

Product Page - Scientific background :

Netrins are a family of highly conserved proteins responsible for axon guidance and cell movement throughout neural development. Netrins can be divided into secreted netrins (netrin 1,3,4 and 5) and membrane-tethered glycophosphatidylinositol (GPI)-linked netrins (netrin G1 and G2)1.Secreted netrins carry their function via interaction with several receptors that include the deleted in colorectal (DCC) family, and the uncoordinated-5 (UNC5-A through UNC5-D) family2.Netrin-3 was discovered in 1997 using sequence homology searching of netrin-2. Similarly to other netrins, Netrin-3 plays an important role in the development of the nervous system. Netrin-3 structure consist of a laminin-like domain located on the N-terminal, three epidermal growth factors like repeats (EGF), and a C-terminal netrin-like domain (NTR). Mutations in NTN3, the gene encoding netrin-3 was found to be associated with the development of several carcinomas3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

NTN3, Netrin-2-Like Protein, NTN2L

Short Description :

A Rabbit Polyclonal antibody to Netrin-3

Long Description :

Anti-Netrin-3 Antibody (#ANR-123) is a highly specific antibody directed against an epitope of the mouse Netrin-3 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Netrin-3 from rat, mouse and human samples.

Negative Control :

BLP-NR123

Positive Control :

Synonyms :

NTN3, Netrin-2-Like Protein, NTN2L

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NTN3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage