Anti-Netrin-1 Antibody | Alomone Labs ANR-121 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Netrin-1 Antibody

catalog :

ANR-121

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4): - 1, 2. Anti-Netrin-1 Antibody (#ANR-121), (1:200). 3, 4. Anti-Netrin-1 Antibody, preincubated with Netrin-1 Blocking Peptide (#BLP-NR121).

image 2 :

Expression of Netrin-1 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Netrin-1 Antibody (#ANR-121), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Netrin-1 immunoreactivity (green) appears in cells (horizontal arrows) in the hilus (H) in the hippocampal dentate gyrus. B. Pre-incubation of the antibody with Netrin-1 Blocking Peptide (BLP-NR121), suppressed staining. Cell nuclei are stained with DAPI (blue). G= granule layer, H = Hilus.

product information

CAT :

ANR-121

SKU :

ANR-121-CF_0.2 ml

Product Name :

Anti-Netrin-1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q924Z9

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR121

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Secreted

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CRKMYNRPHRAPITK, corresponding to amino acid residues 181 - 195 of rat Netrin-1

Product Page - Scientific background :

Netrins are a family comprised of five members: three secreted proteins (netrins 1, 3 and 4), and two glycosylphosphatidylinositol (GPI)-anchored membrane proteins, netrins G1 and G21. Netrin-1 is a diffusible long-range axonal guidance cue expressed in floor plate cells of the central nervous system. It is secreted in order to direct exons to their target1-3. Axon guidance by netrin-1 is implicated in multiple developing brain regions and developing neuronal types. It also plays a role in axon branching, synaptogenesis, cell migration, cell survival, and axon regeneration4.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

NTN1, MRMV4

Short Description :

A Rabbit Polyclonal Antibody to Netrin-1

Long Description :

Anti-Netrin-1 Antibody (#ANR-121) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Netrin-1 from human, mouse, and rat samples.

Negative Control :

BLP-NR121

Positive Control :

Synonyms :

NTN1, MRMV4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NTN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage