Anti-NRG2 (extracellular) Antibody | Alomone Labs ANR-114 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NRG2 (extracellular) Antibody

catalog :

ANR-114

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1,2. Anti-NRG2 (extracellular) Antibody (#ANR-114), (1:200).3,4. Anti-NRG2 (extracellular) Antibody, preincubated with NRG2 (extracellular) Blocking Peptide (#BLP-NR114).

image 2 :

Expression of Neuregulin-2 in mouse hippocampus and cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NRG2 (extracellular) Antibody (#ANR-114), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NRG2 staining in CA1 hippocampal region (green) is detected in the pyramidal layer (P, arrows). B. Staining in the cerebellum (green) is observed in Purkinje cells (arrows). Mol= Molecular layer; GL= Granular layer. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-114

SKU :

ANR-114-CF_0.2 ml

Product Name :

Anti-NRG2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35569

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR114

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RDIRIKYGNGRKNSR, corresponding to amino acid residues 297-311 of rat Neuregulin-2

Product Page - Scientific background :

Neuregulins (NRGs) are a multi member protein family involved in cell to cell communication. This family includes four human neuregulin paralogs NRG1-NRG4 which produce multiple isoforms through alternative splicing.NRGs are involved in several physiological processes including heart and peripheral nervous system development. They act in part by binding to the extracellular region of ErbB/HER transmembrane tyrosine kinases, leading to receptor dimerization and ultimately to the activation of a complex network of downstream signaling events1,2.NRG2 transcript levels markedly increase following birth, suggesting that NRG2 has important functions in the postnatal and adult brain. Several studies have also shown that NRG2 modulates glutamatergic transmission in GABAergic neurons and selectively affects GluN2B-containing NMDA receptors2,3.The region containing the NRG2 locus is associated with schizophrenia and other psychiatric disorders and genetically linked to ADHD in genome-wide scans2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Neuregulin-2, Pro-neuregulin-2 membrane-bound isoform, Pro-NRG2, Neural- and thymus-derived activator for ErbB kinases, NTAK

Short Description :

A Rabbit Polyclonal Antibody to Neuregulin-2

Long Description :

Anti-NRG2 (extracellular) Antibody (#ANR-114) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Neuregulin-2 from rat, mouse, and human samples.

Negative Control :

BLP-NR114

Positive Control :

Synonyms :

Neuregulin-2, Pro-neuregulin-2 membrane-bound isoform, Pro-NRG2, Neural- and thymus-derived activator for ErbB kinases, NTAK

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NRG2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage