Anti-NRG1 Type III (extracellular) Antibody | Alomone Labs ANR-113 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NRG1 Type III (extracellular) Antibody

catalog :

ANR-113

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Gatius A, Tarabal O, Cayuela P, Casanovas A, Piedrafita L, Salvany S, et al. The Y172 Monoclonal Antibody Against p-c-Jun (Ser63) Is a Marker of the Postsynaptic Compartment of C-Type Cholinergic Afferent Synapses on Motoneurons. Front Cell Neurosci. 2019;13:582 pubmed publisher
Jones S, Walton R, Morton M, Lancaster M. K2p 3.1 protein is expressed as a transmural gradient across the rat left ventricular free wall. J Cardiovasc Electrophysiol. 2019;30:383-391 pubmed publisher

image

image 1 :

Western blot analysis of rat dorsal root ganglion lysate: - 1. Anti-NRG1 Type III (extracellular) Antibody (#ANR-113), (1:200).2. Anti-NRG1 Type III (extracellular) Antibody, preincubated with NRG1 Type III (extracellular) Blocking Peptide (#BLP-NR113).

product information

CAT :

ANR-113

SKU :

ANR-113-CF_0.2 ml

Product Name :

Anti-NRG1 Type III (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q02297

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR113

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DKIFEYDSPTHLD, corresponding to amino acid residues 101-113 of human Neuregulin-1 Type III

Product Page - Scientific background :

Neuregulin-1 (NRG1) is a member of a subfamily of the epidermal growth factor family and plays an important role in the development of the peripheral nervous system. Through alternative RNA splicing and promoter usage, the NRG1 gene encodes an entire family of more than 15 transmembrane and secreted isoforms. Neuregulin-1 acts as an important regulator in the survival of Schwann cell precursors, Schwann cell proliferation, motility, axon ensheathment and myelination.Neuregulin-1 can be divided into different according to the N-terminal sequence. Neuregulin-1 Type III, a transmembrane protein, contains an epidermal growth factor-like domain, which alone is adequate to bind and activate ErbB receptor tyrosine kinases. Type III also contains a cysteine-rich domain called "cysteine-rich domain-neuregulins," that requires a proteolytic cleavage for full activity1,2.Knockout studies show that type III is the key isoform required for schwann cell generation. These studies also confirmed that type III isoform is necessary for promoting myelination and to regulate myelin thickness1,2. Several different studies also showed that NRG1 Type III isoform is also an important regulator of cholinergic signaling. Neuregulin-1 Type III regulates the expression of AChR genes and targeting of nAChRs to the neuronal cell surface. This isoform in particular is a critical regulator of presynaptic targeting of α7-containing nAChRs3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Neuregulin-1 isoform 10, CRD-NRG, Sensory and motor neuron derived factor, SMDF

Short Description :

A Rabbit Polyclonal Antibody to Neuregulin-1 Type III

Long Description :

Anti-NRG1 Type III (extracellular) Antibody (#ANR-113) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NRG1 Type III from rat, human and mouse samples. The antibody is specific for NRG1 Type III and does not recognize other Neuregulin-1 isoforms.

Negative Control :

BLP-NR113

Positive Control :

Synonyms :

Neuregulin-1 isoform 10, CRD-NRG, Sensory and motor neuron derived factor, SMDF

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NRG1

AB Specifictiy :

This antibody will recognize Neuregulin-1 Type III. It will not recognize Neuregulin-1 Type I, Type II and Type IV.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage