Anti-NRG1 (Neuregulin-1) (extracellular) Antibody | Alomone Labs ANR-111 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NRG1 (Neuregulin-1) (extracellular) Antibody

catalog :

ANR-111

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat heart lysate (lanes 1 and 4), mouse heart membranes (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6): - 1-3. Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (#ANR-111), (1:200).4-6. Anti-NRG1 (Neuregulin-1) (extracellular) Antibody, preincubated with NRG1/Neuregulin-1 (extracellular) Blocking Peptide (#BLP-NR111).

image 2 :

Expression of Neuregulin-1 in rat neocortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (#ANR-111), (1:1000), followed by anti-rabbit-biotinylated antibody and streptavidin-Cy3 (red). Neuregulin-1 staining is strong in processes of various cell types (horizontal arrows) and less intense in neuron-shaped soma (vertical arrows). Nuclei are stained with DAPI (blue).

product information

CAT :

ANR-111

SKU :

ANR-111-CF_0.2 ml

Product Name :

Anti-NRG1 (Neuregulin-1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q02297

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR111

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KIQKKPGKSELRINK, corresponding to amino acid residues 87-101 of human Neuregulin-1

Product Page - Scientific background :

ErbB receptors are a family of tyrosine kinase receptors encoded by the erbB gene. These receptors commence signaling pathways such as the mitogen activated protein kinase (MAPK) and inositol 3-kinase (PI3K) pathways and have an important role in the control of cell growth and survival1. Neuregulins are a family of EGF-like factors that are produced from alternative RNA splicing of the same gene. Through their interaction with ErbB receptors, Neuregulins regulate normal developmental processes, plasticity, and oncogenesis2.Neuregulin-1 is one of the four known proteins of the Neuregulin family and can be found in many organs including the heart, breast, peripheral and central nervous system. Neuregulin-1 is encoded by a gene located on chromosome 8p13 which produces more than 15 different isoforms of the same protein. All isoforms contain a core EGF domain encoded by exon E130 but other elements of the protein vary. Most NRG1 isoforms are synthesized as transmembrane proproteins which undergo proteolytic cleavage processes3.Due to its important function in the central and peripheral nervous system as well as in other organs NRG1 has been implicated in a variety of pathologies. The NRG1 gene is suspected as a schizophrenia susceptibility gene. Mice with a mutated NRG1 exhibit elevated activity in open field and abnormal sensorimotor gating which correspond with schizophrenic like behavior in humans. These affects are reversed using anti-psychotic drugs4. In addition, Activation of NRG1 was found to improve cardiac survival and function in a rat and canine model of dilated and ischemic hearts5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF

Short Description :

A Rabbit Polyclonal Antibody to Neuregulin-1

Long Description :

Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (#ANR-111) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and could potentially detect the protein in living cells. It has been designed to recognize Neuregulin-1 from rat, human, and mouse samples. This antibody will recognize NRG1 Type I, Type II and Type IV. It will not recognize Neuregulin-1 Type III.

Negative Control :

BLP-NR111

Positive Control :

Synonyms :

Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NRG1

AB Specifictiy :

This antibody will recognize Neuregulin-1 Type I, Type II and Type IV. It will not recognize Neuregulin-1 Type III.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage