antibody

Anti-NRG1 (Neuregulin-1) (extracellular)-ATTO Fluor-488 Antibody

ANR-111-AG

polyclonal

domestic rabbit

ATTO 488

NA

immunohistochemistry, immunocytochemistry

ANR-111-AG_50 mcl

Anti-NRG1 (Neuregulin-1) (extracellular)-ATTO Fluor-488 Antibody

Antibodies

Antibodies

Polyclonal

Q02297

IF IHC

Mouse

Rabbit

BLP-NR111

Mouse, human - identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Extracellular, N-terminus

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

(C)KIQKKPGKSELRINK, corresponding to amino acid residues 87-101 of human Neuregulin-1

ErbB receptors are a family of tyrosine kinase receptors encoded by the erbB gene. These receptors commence signaling pathways such as the mitogen activated protein kinase (MAPK) and inositol 3-kinase (PI3K) pathways and have an important role in the control of cell growth and survival1. Neuregulins are a family of EGF-like factors that are produced from alternative RNA splicing of the same gene. Through their interaction with ErbB receptors, Neuregulins regulate normal developmental processes, plasticity, and oncogenesis2.Neuregulin-1 is one of the four known proteins of the Neuregulin family and can be found in many organs including the heart, breast, peripheral and central nervous system. Neuregulin-1 is encoded by a gene located on chromosome 8p13 which produces more than 15 different isoforms of the same protein. All isoforms contain a core EGF domain encoded by exon E130 but other elements of the protein vary. Most NRG1 isoforms are synthesized as transmembrane proproteins which undergo proteolytic cleavage processes3.Due to its important function in the central and peripheral nervous system as well as in other organs NRG1 has been implicated in a variety of pathologies. The NRG1 gene is suspected as a schizophrenia susceptibility gene. Mice with a mutated NRG1 exhibit elevated activity in open field and abnormal sensorimotor gating which correspond with schizophrenic like behavior in humans. These affects are reversed using anti-psychotic drugs4. In addition, Activation of NRG1 was found to improve cardiac survival and function in a rat and canine model of dilated and ischemic hearts5.

IF IHC

Alomone Labs

Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF

A Rabbit Polyclonal Antibody to Neuregulin-1 Conjugated to the Fluorescent Dye ATTO-488

Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (#ANR-111) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and could potentially detect the protein in living cells. It has been designed to recognize Neuregulin-1 from rat, human, and mouse samples. This antibody will recognize NRG1 Type I, Type II and Type IV. It will not recognize Neuregulin-1 Type III. Anti-NRG1 (Neuregulin-1) (extracellular)-ATTO Fluor-488 Antibody (#ANR-111-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-NRG1 (Neuregulin-1) (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

RIC-001-AG

NA

Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

NRG1

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #ANR-111), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

1:80

Contact Alomone for information