Anti-Parkin Antibody | Alomone Labs ANR-103 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Parkin Antibody

catalog :

ANR-103

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain synaptosome preparation (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1-2. Anti-Parkin Antibody (#ANR-103), (1:200). 3-4. Anti-Parkin Antibody, preincubated with Parkin Blocking Peptide (BLP-NR103).

image 2 :

Expression of Parkin in rat brain 4th ventricle. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Parkin Antibody (#ANR-103), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Parkin immunoreactivity (green) appears along the wall of the 4th cerebral ventricle (arrows). B. Pre-incubation of the antibody with Parkin Blocking Peptide (BLP-NR103), suppressed staining. Cell nuclei are stained with DAPI (blue). 4th V= 4th ventricle.

product information

CAT :

ANR-103

SKU :

ANR-103-CF_0.2 ml

Product Name :

Anti-Parkin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WVS6

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR103

Homology :

Rat, Human - 15 out of 16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KELHHFRILGEEQYTR, corresponding to amino acid residues 298 - 313 of mouse Parkin

Product Page - Scientific background :

Protein degradation is a major process in the cell, allowing control and adjustment of the levels of proteins. The enzyme responsible for the vast majority of cellular protein degradation is the 26S proteasome1. Protein degradation is a highly regulated process that dependents on specific poly-ubiquitination of the substrate. The ubiquitination pathway is catalyzed by E1, E2 and E3 enzymes that activate, conjugate, and ligate, respectively, the ubiquitin protein to the substrate protein2.Parkin is a cytosolic E3 ubiquitin ligase that plays an important role in neuroprotection. Structurally, the Parkin protein is comprised of a ubiquitin-like (Ubl) domain at its N-terminus and a really interesting new gene (RING)1-in-between-ring (IBR) -RING2 domain and a zinc-chelating (RING0) domain at its C-terminus. E2 ubiquitin-conjugated enzymes are recruited to the RING1 domain where they transfer ubiquitin to the RING2 domain at the Parkin catalytic site. The ubiquitin is then transferred to the substrate protein, where it serves as a tag for recognition by the proteasome3.Parkin activity prevent the accumulation and aggregation of damaged proteins and therefore it protects against unfolded protein stress. In addition, it is known to promote the elimination of aberrant mitochondria via mitophagy4. Mutations in Parkin have been associated with Parkinson's disease (PD), Alzheimer's disease and Huntington's disease5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

E3 Ubiquitin-Protein Ligase Parkin, Parkin RBR E3 ubiquitin-protein ligase, Parkinson Juvenile Disease Protein 2, PARK2, PRKN

Short Description :

A Rabbit Polyclonal antibody to Parkin

Long Description :

Anti-Parkin Antibody (#ANR-103) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Parkin from rat, mouse and human samples.

Negative Control :

BLP-NR103

Positive Control :

Synonyms :

E3 Ubiquitin-Protein Ligase Parkin, Parkin RBR E3 ubiquitin-protein ligase, Parkinson Juvenile Disease Protein 2, PARK2, PRKN

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PRKN

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage