Anti-LRRK2 Antibody | Alomone Labs ANR-102 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LRRK2 Antibody

catalog :

ANR-102

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Sizemore G, McLaughlin S, Newman M, Brundage K, Ammer A, Martin K, et al. Opening large-conductance potassium channels selectively induced cell death of triple-negative breast cancer. BMC Cancer. 2020;20:595 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-LRRK2 Antibody (#ANR-102), (1:200).3,4. Anti-LRRK2 Antibody, preincubated with LRRK2 Blocking Peptide (#BLP-NR102).

image 2 :

Expression of LRRK2 in rat striatum and substantia nigra - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-LRRK2 Antibody (#ANR-102), (1:400), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. A. LRKK2 staining (green) of striatum sections is detected in the cytoplasm of several striatal cells (arrows). B. Staining in the substantia nigra pars compacta shows LRKK2 staining (green) in nuclei and cytoplasm of several cells (arrows). Nuclei were stained with DAPI (blue).

product information

CAT :

ANR-102

SKU :

ANR-102-CF_0.2 ml

Product Name :

Anti-LRRK2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q5S006

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR102

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CKALHVLFERVSEE, corresponding to amino acid residues 183-196 of mouse LRRK2

Product Page - Scientific background :

Parkinson's disease (PD) is the second most common neurodegenerative disease. The condition is inherited in 5-10% of all PD patients. Mutations in the PARK8 gene have been implicated in the development of PD.PARK8 gene encodes the protein LRRK2 (Leucine-rich repeat kinase 2). It is a multi-domain protein containing distinctive enzymatic domains of GTPase and a kinase, the protein interaction motifs LRR (Leucine-rich repeat) and WD40. LRRK2's domain structure indicates that it belongs to the Roco protein family. LRRK2 is expressed in the kidney, lung and dopaminergic neurons in the substantia nigra. It is mostly located in the cytosol, however, it is also expressed on membranes of organelles such as the mitochondria, golgi apparatus and synaptic vesicles.There are several PARK8 mutations that have been associated with both familial and sporadic PD. The most common mutation, G2019S, is present in 85% of PD patients carrying PARK8 mutations and in 4% of all familial PD patients. In addition, this mutation has been identified in 1% of sporadic PD patients. The prevalence of this mutation is especially high in Ashkenazi Jewish and North-African Arab populations. This mutation causes increased kinase activity while other mutations, such as R1441C, decrease GTPase activity. Overexpression of the LRRK2 protein causes neuronal cell death and other pathological conditions which are aggravated by the overexpression of mutant proteins.The exact role of LRRK2 in PD pathophysiology still remains unclear1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Leucine-rich repeat kinase 2, Parkinson disease (Autosomal dominant) 8, PARK8, Roco2, RIPK7, Dardarin

Short Description :

A Rabbit Polyclonal Antibody to LRRK2

Long Description :

Anti-LRRK2 Antibody (#ANR-102) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize LRKK2 from human, mouse, and rat samples.

Negative Control :

BLP-NR102

Positive Control :

Synonyms :

Leucine-rich repeat kinase 2, Parkinson disease (Autosomal dominant) 8, PARK8, Roco2, RIPK7, Dardarin

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LRRK2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage