Anti-LRRK1 Antibody | Alomone Labs ANR-101 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LRRK1 Antibody

catalog :

ANR-101

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Kang J, Huang G, Ma L, Tong Y, Shahapal A, Chen P, et al. Cell autonomous role of leucine-rich repeat kinase in protection of dopaminergic neuron survival. bioRxiv. 2023;: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-LRRK1 Antibody (#ANR-101), (1:200).3,4. Anti-LRRK1 Antibody, preincubated with LRRK1 Blocking Peptide (#BLP-NR101).

image 2 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)brain lysates: - 12.Anti-LRRK1 Antibody (#ANR-101) (1:200).34. Anti-LRRK1 Antibody preincubated with the negative control antigen.

product information

CAT :

ANR-101

SKU :

ANR-101-CF_0.2 ml

Product Name :

Anti-LRRK1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q3UHC2

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR101

Homology :

Rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EIHTAYKQRNLSRAR, corresponding to amino acid residues 57-71 of mouse LRRK1

Product Page - Scientific background :

Leucine-rich repeat kinase 1 (LRRK1) is a member of the ROCO protein kinase superfamily.ROCO is a family of multidomain proteins characterized by the presence of tandem ROC (Ras of Complex Proteins) and COR (C-terminal of ROC) domains. In humans, ROCO proteins comprises four members: Leucine-rich repeat kinase 1 (LRRK1), Leucine-rich repeat kinase 2 (LRRK2), Death-associated kinase 1 (DAPK1) and Malignant fibrous histiocytoma amplified sequence 1 (MFASH1). In addition, LRRK1 contains a GTPase domain and MAPKKK-like kinase domain. LRRK1 structure includes a serine-threonine kinase domain C-terminal of ROC-COR, and leucine-rich and ankyrin-like repeats at the N-terminus1.LRRK1 can form hetero- and homo-dimers and it seems that the dimer is the functional unit of the protein. LRRK1 kinase activity is stimulated by GTP binding to the Roc domain and the protein is basally phosphorylated1.LRRK1 is involved in the trafficking of activated epidermal growth factor receptor (EGFR). The N-terminus of LRRK1 interacts with Grb2, an adaptor protein for EGFR, and forms a complex with EGFR via Grb2 in response to EGF stimulation2.Data suggest that variants in LRRK1 in addition to LRRK2, may have a role in the genetic foundation of Parkinson's disease3. Also, the dimerization of LRRK1 with LRRK2 may influence the age of symptomatic expression in LRRK2-parkinsonism patients4.LRRK1 knockout mice provide evidence that LRRK1 has an important role in negative regulation of bone mass in part through modulating the c-Src signaling pathway in mice5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Leucine-rich repeat kinase 1, Roco1, RIPK6

Short Description :

A Rabbit Polyclonal Antibody to LRRK1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse LRRK1. Anti-LRRK1 Antibody (#ANR-101) can be used in western blot analysis. It has been designed to recognize LRRK1 from rat and mouse samples. The antibody is not recommended to be used on human samples.

Negative Control :

BLP-NR101

Positive Control :

Synonyms :

Leucine-rich repeat kinase 1, Roco1, RIPK6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LRRK1

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage