Anti-SV2A Antibody | Alomone Labs ANR-095 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SV2A Antibody

catalog :

ANR-095

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):

image 2 :

Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human ARPE-19 retinal pigmental epithelia cell line lysate (lanes 2 and 4):

image 3 :

Expression of SV2A in mouse hippocampus

product information

CAT :

ANR-095

SKU :

ANR-095-CF_0.2 ml

Product Name :

Anti-SV2A Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JIS5

Applications :

IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR095

Homology :

Rat, human- identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KGERMADGAPLAGVR, corresponding to amino acid residues 109 - 123 of mouse SV2A

Product Page - Scientific background :

SV2A, also known as Synaptic vesicle glycoprotein 2A and Synaptic vesicle protein 2A, is an integral membrane glycoprotein located on synaptic vesicles, which are responsible for storing and releasing neurotransmitters in the brain.1,2SV2A is a member of a protein family that also includes SV2B and SV2C. All three proteins exhibit a similar topology of 12 transmembrane domains, but have different expression patterns in the brain. SV2A is ubiquitously expressed and is found in virtually all types of neurons throughout the brain, while SV2B has a more restricted expression pattern, being present in certain areas of the brain such as the hippocampus and cortex. SV2C displays a more limited expression, and is primarily found in the basal ganglia and some brainstem nuclei.1,2The exact physiological role of SV2A is not clear, but a role in the regulation of neurotransmitter release, maintenance of synaptic vesicle pool and modulation of synaptic plasticity, have been suggested. 1,3Interestingly, the role of SV2A in pathological conditions, has received considerable attention. Animals homozygous for the SV2A gene appear normal at birth, however, they fail to grow, experience severe seizures, and die within 3 weeks. 4In fact, SV2A was identified as the binding site for the antiepileptic drug levetiracetam, which is effective in reducing seizure frequency in epilepsy patients.1,3In addition, changes in SV2A expression have been observed in neurodegenerative diseases like Alzheimer's, schizophrenia, Parkinson's disease and dementia. 1,3Changes in SV2A expression are increasingly recognized as a valuable biomarker for synaptic density, a parameter providing insights into the integrity and function of synapses in the brain. Synaptic density refers to the number of synapses in a given volume of brain tissue and is a critical indicator of neuronal health and connectivity. For this purpose, radioligands that specifically bind to SV2A and can be used in Positron Emission Tomography (PET) scans, have been developed to quantify synaptic density non-invasively. Thus, measuring SV2A expression is a promising tool for diagnosing and monitoring neurological and psychiatric conditions, ultimately aiding in the development of more effective treatments.1,5,6

Applications may also work in :

IHC IP WB

Supplier :

Alomone Labs

Target :

Synaptic vesicle glycoprotein 2A, Synaptic vesicle protein 2A

Short Description :

A Rabbit Polyclonal Antibody to SV2A

Long Description :

Anti-SV2A Antibody (#ANR-095) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation and immunohistochemistry applications. It has been designed to recognize SV2A from rat, mouse and human samples.

Negative Control :

BLP-NR095

Positive Control :

Synonyms :

Synaptic vesicle glycoprotein 2A, Synaptic vesicle protein 2A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SV2A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage