Anti-Periaxin Antibody | Alomone Labs ANR-091 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Periaxin Antibody

catalog :

ANR-091

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat DRG (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: - 1,2. Anti-Periaxin Antibody (#ANR-091), (1:600).3,4. Anti-Periaxin Antibody, preincubated with Periaxin Blocking Peptide (#BLP-NR091).

image 2 :

Expression of Periaxin in rat peripheral nerve - Immunohistochemical staining of free floating frozen rat peripheral nerve sections using Anti-Periaxin Antibody (#ANR-091), (#1:600). A. PRX staining (green) appears in a peripheral nerve (arrow) connecting to the spinal dorsal horn (D horn). Note the absence of staining of spinal myelin column indicating the specificity of the antibody to peripheral myelin. B. A higher magnification of the insert from panel A. C. Segment of peripheral nerve contains PRX-positive fibers. (arrows). In all panels DAPI is used as the counterstain (blue).

product information

CAT :

ANR-091

SKU :

ANR-091-CF_0.2 ml

Product Name :

Anti-Periaxin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63425

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR091

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DVEFSFPKFSRLRR, corresponding to amino acid residues 155-170 of rat PRX

Product Page - Scientific background :

Periaxin protein is a membrane-associated protein expressed in myelinating Schwann cells. It is required for the stable maintenance and development of a normal myelin sheath in the peripheral nervous system. It is also a member of cytoskeletal complexes in lens fibers where it plays an important role in maturation, packing, and membrane organization of lens fiber cell1,2. The periaxin gene (PRX) encodes two protein isoforms, L-periaxin and S-periaxin generated by alternative mRNA splicing. These proteins contain N-terminal PDZ domains (PSD-95/Discs-large/ZO-1) important for organizing protein-protein interactions.L-periaxin is a component of the dystroglycan-dystrophin-related protein-2 complex (DRP2) linking the Schwann cell cytoskeleton to the extracellular matrix. Its location changes during myelination; at first the protein is localized to the adaxonal plasma membrane and later, in the abaxonal plasma membrane. S-periaxin is distributed in a uniform manner in the cytoplasm and the nucleus of the Schwann cell1-3. Mutations in the Periaxin gene are known to cause autosomal recessive demyelinating in Type 4F Charcot-Marie-Tooth (CMT4F) and Dejerine-Sottas disease3.Periaxin knockout mice develop normally myelinated peripheral nervous system at first but develop a late onset demyelinating peripheral neuropathy1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

PRX, CMT4F

Short Description :

A Rabbit Polyclonal Antibody to Periaxin

Long Description :

Anti-Periaxin Antibody (#ANR-091) is a highly specific antibody directed against an epitope of rat PRX. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PRX from mouse, rat and human samples. The antibody specifically recognizes L-Periaxin. It does not recognize S-Periaxin.

Negative Control :

BLP-NR091

Positive Control :

Synonyms :

PRX, CMT4F

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PRX

AB Specifictiy :

Specific to isoform 1 also known as L-Periaxin (will not recognize isoform 2 known as S-Periaxin).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage