Guinea pig Anti-Periaxin Antibody | Alomone Labs ANR-091-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-Periaxin Antibody

catalog :

ANR-091-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Delgado C, Bu L, Zhang J, Liu F, Sall J, Liang F, et al. Neural cell adhesion molecule is required for ventricular conduction system development. Development. 2021;148: pubmed publisher

image

image 1 :

Western blot analysis of rat DRG lysate: - 1. Guinea pig Anti-Periaxin Antibody (#ANR-091-GP), (1:200). 2. Guinea pig Anti-Periaxin Antibody, preincubated with Periaxin Blocking Peptide (#BLP-NR091).

image 2 :

Expression of Periaxin in rat spinal dorsal root entry zone - Immunohistochemical staining of rat spinal dorsal root entry zone frozen sections using Guinea pig Anti-Periaxin Antibody (#ANR-091-GP). Periaxin immunoreactivity (red) appears in the dorsal nerve root (arrows). Nuclei are stained using DAPI as the counterstain (blue).

product information

CAT :

ANR-091-GP

SKU :

ANR-091-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-Periaxin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63425

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-NR091

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DVEFSFPKFSRLRR, corresponding to amino acid residues 155-170 of rat PRX

Product Page - Scientific background :

Periaxin protein is a membrane-associated protein expressed in myelinating Schwann cells. It is required for the stable maintenance and development of a normal myelin sheath in the peripheral nervous system. It is also a member of cytoskeletal complexes in lens fibers where it plays an important role in maturation, packing, and membrane organization of lens fiber cell1,2. The periaxin gene (PRX) encodes two protein isoforms, L-periaxin and S-periaxin generated by alternative mRNA splicing. These proteins contain N-terminal PDZ domains (PSD-95/Discs-large/ZO-1) important for organizing protein-protein interactions.L-periaxin is a component of the dystroglycan-dystrophin-related protein-2 complex (DRP2) linking the Schwann cell cytoskeleton to the extracellular matrix. Its location changes during myelination; at first the protein is localized to the adaxonal plasma membrane and later, in the abaxonal plasma membrane. S-periaxin is distributed in a uniform manner in the cytoplasm and the nucleus of the Schwann cell1-3. Mutations in the Periaxin gene are known to cause autosomal recessive demyelinating in Type 4F Charcot-Marie-Tooth (CMT4F) and Dejerine-Sottas disease3.Periaxin knockout mice develop normally myelinated peripheral nervous system at first but develop a late onset demyelinating peripheral neuropathy1.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

PRX, CMT4F

Short Description :

A Guinea Pig Polyclonal Antibody to Periaxin

Long Description :

Guinea pig Anti-Periaxin Antibody (#ANR-091-GP, formerly AGP-215), raised in guinea pig, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Periaxin from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Periaxin Antibody (#ANR-091) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-NR091

Positive Control :

Synonyms :

PRX, CMT4F

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PRX

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:600

more info or order :

Alomone Labs product webpage