Anti-Neuroplastin (extracellular) Antibody | Alomone Labs ANR-090 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neuroplastin (extracellular) Antibody

catalog :

ANR-090

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 4), rat cerebellum lysate (lanes 2 and 5) and mouse brain membrane (lanes 3 and 6): - 1-3. Anti-Neuroplastin (extracellular) Antibody (#ANR-090), (1:400).4-6. Anti-Neuroplastin (extracellular) Antibody, preincubated with Neuroplastin (extracellular) Blocking Peptide (#BLP-NR090).

image 2 :

Expression of Neuroplastin in rat hippocampus and rat cortex - Immunohistochemical staining of free floating frozen section of rat hippocampal CA1 region (A) and posterior cingulate cortex (B) using Anti-Neuroplastin (extracellular) Antibody (#ANR-090), (1:120). A. NPTN staining (green) appears in neurons (vertical arrows) of the pyramidal layer (P) and in apical dendrites (horizontal arrows). B. NPTN staining (green) is detected in pyramidal neurons in the soma (vertical arrows) and in their apical dendrites (diagonal arrows). Nuclei were stained using DAPI as the counterstain (blue).

product information

CAT :

ANR-090

SKU :

ANR-090-CF_0.2 ml

Product Name :

Anti-Neuroplastin (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97546

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR090

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RKNASNMEYRINKPRAE, corresponding to amino acid residues 194-210 of rat NPTN

Product Page - Scientific background :

The neuroplastins Np55 and Np65 comprise a superfamily of neuronal and synapse enriched immunoglobulin molecules. They play an important role in a number of vital synaptic and neuronal activities including cell adhesion in the case of Np65. Np65 and Np55 are identical in structure except for the N-terminal Np65-specific Ig1 domain. They also play a key role in the localization of GABA(A) receptors in inhibitory synapses.Neuroplastins have two Ig domains that project into the extracellular space and are followed by a single 21 amino-acid membrane spanning sequence and a 34 amino-acid intracellular domain. The intracellular domain is mostly comprised of charged and neutral residues but also of a few hydrophobic residues. The N-terminus includes a 28 amino-acid signal peptide. In addition, neuroplastins contain a glutamate residue in their transmembrane domain which is important for molecular interactions within the membrane region.Although Np55 and Np65 are closely related in structure there are significant differences in terms of their expression. Np65 is tissue specific and is expressed mostly in forebrain neurons in the cortex, hippocampus and striatum. It has an anterior-posterior axis of expression. It is also expressed in lower amounts in the thalamus and hypothalamus. In contrast, Np55 is expressed throughout the brain in varying amounts.Currently, neuroplastins are not directly implicated in human diseases however several possible links have been discovered. A single nucleotide polymorphism (SNP) in the human neuroplastin locus is associated with reduced cortical thickness and impaired intellectual ability in adolescents. Furthermore, other SNPs in neuroplastin has been found to be either a risk or protecting factor for schizophrenia in animal models1.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

NPTN, Glycoprotein 55/65, GP55, GP65, Np55, Np65, Stromal cell-derived receptor 1, SDR1, SDFR1

Short Description :

A Rabbit Polyclonal Antibody to Neuroplastin

Long Description :

Anti-Neuroplastin (extracellular) Antibody (#ANR-090) is a highly specific antibody designed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting NPTN in living cells. It has been designed to recognize NPTN from rat, mouse and human samples.

Negative Control :

BLP-NR090

Positive Control :

Synonyms :

NPTN, Glycoprotein 55/65, GP55, GP65, Np55, Np65, Stromal cell-derived receptor 1, SDR1, SDFR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NPTN

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:120

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage