Anti-N-Cadherin Antibody | Alomone Labs ANR-082 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-N-Cadherin Antibody

catalog :

ANR-082

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 5), mouse heart (lanes 2 and 6), rat heart (lanes 3 and 7) and human brain neuroblastoma (SH-SY5Y) (lanes 4 and 8) cell lysates: - 1-4. Anti-N-Cadherin Antibody (#ANR-082), (1:200).5-8. Anti-N-Cadherin Antibody, preincubated with N-Cadherin Blocking Peptide (#BLP-NR082).

image 2 :

Expression of N-Cadherin in mouse cortex - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-N-Cadherin Antibody (#ANR-082), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. N-Cadherin immunoreactivity (green) appears in apical dendrites (horizontal arrows) and in the soma (vertical arrows) of pyramidal neurons. B. Pre-incubation of the antibody with N-Cadherin Blocking Peptide (BLP-NR082), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-082

SKU :

ANR-082-CF_0.2 ml

Product Name :

Anti-N-Cadherin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P15116

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR082

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)DTVEPDAIKPVGIR, corresponding to amino acid residues 794-807 of mouse CDH2

Product Page - Scientific background :

Cadherins are transmembrane proteins responsible for cell adhesion. Cell adhesion is a crucial feature for the sustainability and maintenance of normal tissue function and three dimensional structure. Cadherins share an extracellular domain consisting of multiple repeats of cadherin-specific motif and are calcium dependent homotypic cell-to-cell adhesion molecules. They are localized mostly in specialized sites called adherence junctions1.N-cadherin consists of an amino-terminal external domain with five tandem repeats, a single transmembrane segment, and a cytoplasmic carboxy-terminal domain of approximately 150 amino acids. The intracellular domain of N-cadherin interacts with a group of proteins called catenins that are essential for cadherin-mediated cell adhesion. It is suggested that N-cadherin proteins align in a form of "zipper" when involved in cell adhesion. Cadherins on one cell surface form a series of rigid dimers that attach to equivalent dimers on the opposing cells and lateral motion of these complexes allows the cell junction site to "zip up"1.N-cadherin plays a role in tumor metastasis. Unlike E-cadherin that causes tumor metastasis when insufficiently, N-cadherin is involved in tumor metastasis when upregulated. N-cadherin stimulates migration, invasion and metastasis of squamous cell breast cancer and its effects are increased by FGF-2 suggesting that N-cadherin and FGFR-1 synergize and promote aggressive tumor behavior. This synergy also promotes neuronal growth1. Interestingly, low levels of N-cadherin are associated with a higher invasive capacity of astrocytic glioma by increasing tumor (and normal) cell migration speed and creating a less organized pattern of migration3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal

Short Description :

A Rabbit Polyclonal Antibody to N-Cadherin

Long Description :

Anti-N-Cadherin Antibody (#ANR-082) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize CDH2 from rat, mouse, and human samples.

Negative Control :

BLP-NR082

Positive Control :

Synonyms :

Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CDH2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage