Anti-NPBWR1 Antibody | Alomone Labs ANR-075 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NPBWR1 Antibody

catalog :

ANR-075

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysates (lanes 2 and 5) and rat heart lysate (lanes 3 and 6):

image 2 :

Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):

image 3 :

Expression of NPBWR1 in mouse hippocampus.

product information

CAT :

ANR-075

SKU :

ANR-075-CF_0.2 ml

Product Name :

Anti-NPBWR1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49681

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR075

Homology :

Rat - identical; human - 12 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ESRRVSGRTYGAAR, corresponding to amino acid residues 145 - 158 of mouse NPBWR1

Product Page - Scientific background :

Neuropeptides B/W receptor type 1 (NPBWR1), also known as G-protein coupled receptor 7 (GPR7), is a member of the G-protein coupled receptor (GPCR) superfamily.NPBWR1 mediates the biological activities of Neuropeptide B (NPB) and neuropeptide W (NPW) that are structurally and functionally related endogenous neuropeptides. In humans, a second receptor termed NPBWR2 or GPR8, has been identified, however, NPBWR2 is absent in rodents.1,2NPBWR1 is expressed in the central nervous system (CNS) and in peripheral tissues. In the brain expression of NPBWR1 is found in several regions including hypothalamus, hippocampus and amygdala. Indeed, the distribution of NPBWR1 and its ligands in brain is in accordance to their established functions in appetite control and feeding behavior, as well as locomotion, analgesia and stress response.2,3Several studies have pointed to the role of NPBWR1 in pain modulation. Interestingly, NPBWR1 has structural similarities with the opioid receptors and in fact, it binds non-selective opioid ligands such as β-endorphin.3 It has been suggested, that a combination therapies of morphine (an opioid receptor ligand) and NPBWR1 agonists could provide a new avenue to treat inflammatory and neuropathic pain with less risk of adverse effects. 4In the periphery, NPBWR1 may be involved in energy homeostasis by regulating insulin secretion from pancreatic β cells and regulating white and brown adipose tissue. 3

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Neuropeptides B/W Receptor Type 1, G Protein-Coupled Receptor 7, GPR7

Short Description :

A Rabbit Polyclonal antibody to NPBWR1 receptor

Long Description :

Anti-NPBWR1 Antibody (#ANR-075) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NPBWR1 from rat, mouse and human samples.

Negative Control :

BLP-NR075

Positive Control :

Synonyms :

Neuropeptides B/W Receptor Type 1, G Protein-Coupled Receptor 7, GPR7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NPBWR1

AB Specifictiy :

The antibody is specific for NPBWR1, it will not recognize the human only receptor NPBWR2.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage