Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody | Alomone Labs ANR-074 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody

catalog :

ANR-074

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and rat dorsal root ganglion lysate (lanes 2 and 4):

image 2 :

Western blot analysis of mouse Neuro-2a neuroblastoma cell line lysate (lanes 1 and 4), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):

image 3 :

Cell surface detection of NPFFR2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:

product information

CAT :

ANR-074

SKU :

ANR-074-CF_0.2 ml

Product Name :

Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q924H0

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR074

Homology :

Rat - identical Human - 12 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EEKYYRVRLSSHNK, corresponding to amino acid residues 186 - 199 of mouse NPFFR2

Product Page - Scientific background :

Neuropeptide FF receptor 2, NPFFR1, and also known as GPR74, is a member of the G protein-coupled receptor superfamily containing two subtypes, NPFFR1 and NPFFR2.1-2NPFFR1 and NPFFR2 are the high affinity receptors the endogenous neuropeptides NPFF and NPAF, also known as RFamide peptides, which are characterized by their Arg-Phe-NH2 motif at the C-terminal end.1-2NPFFR2 is expressed in both the central and peripheral nervous system, as well as in other organs such as heart, kidney and adipose tissue macrophages.1-4NPFFR2 has been extensively studied for its role in pain modulation. Mice over-expressing NPFFR2 showed more sensitivity to mechanical and thermal noxious stimuli. In addition, a role in the modulation of the development of opioid tolerance and the regulation of withdrawal symptoms, has been suggested.3,5NPFFR2 signaling has been implicated in the regulation of energy homeostasis, through the inhibition of food intake and the modulation of metabolic rate and energy expenditure.2,3,6 In this regard, it is interesting to note that, NPFFR2 has been identified in adipose tissue macrophages, cells that are involved in the development of obesity-induced metabolic diseases.7

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

NPFFR2, G-Protein Coupled Receptor 74, GPR74

Short Description :

A Rabbit Polyclonal antibody to Neuropeptide FF Receptor 2 (extracellular)

Long Description :

Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize NPFFR2 from mouse, rat and human samples.

Negative Control :

BLP-NR074

Positive Control :

Synonyms :

NPFFR2, G-Protein Coupled Receptor 74, GPR74

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NPFFR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage