Anti-NMUR2 (extracellular) Antibody | Alomone Labs ANR-072 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NMUR2 (extracellular) Antibody

catalog :

ANR-072

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Ambite I, Butler D, Stork C, Gr nberg Hern ndez J, K ves B, Zdziarski J, et al. Fimbriae reprogram host gene expression - Divergent effects of P and type 1 fimbriae. PLoS Pathog. 2019;15:e1007671 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human brain glioblastoma (U-87 MG) (lanes 3 and 7) and mouse brain glioma (C6) cell (lanes 4 and 8) lysates: - 1-4. Anti-NMUR2 (extracellular) Antibody (#ANR-072), (1:200).5-8. Anti-NMUR2 (extracellular) Antibody, preincubated with NMUR2 (extracellular) Blocking Peptide (#BLP-NR072).

image 2 :

Cell surface detection ofPAR-1 in live intact human MEG-01 megakaryoblastic leukemia cells: - ___Cells.___Cells + rabbit IgG isotype control-FITC___Cells + Anti-Human PAR1 (F2R) (extracellular)-FITC Antibody(#APR-031-F) (5 g).

product information

CAT :

ANR-072

SKU :

ANR-072-CF_0.2 ml

Product Name :

Anti-NMUR2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9ESQ4

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR072

Homology :

Mouse -13/14 amino acid residues identical; human - 7/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KLENASWIHDPLMK, corresponding to amino acid residues 3-16 of rat NMUR2

Product Page - Scientific background :

Neuromedin U (NMU) is a highly conserved, ubiquitously distributed neuropeptide, which regulates food intake and body weight. Two high-affinity receptors have been identified for NMU: Neuromedin U receptor 1 (NMUR1) and Neuromedin U receptor 2 (NMUR2) that belong to the G-protein coupled receptors (GPCRs). These receptors are members of the rhodopsin-like class A GPCR family and share about 50% identity with each other in the seven transmembrane region.NMUR2 is highly expressed in the central nervous system, in the paraventricular nucleus of the hypothalamus, nuclei accumbens, hippocampus, thalamus, medulla oblongata, and spinal cord. It is also detected in organs such as the uterus and ovary, and is abundant in nociceptive sensory pathways, including spinal dorsal horn, dorsal root ganglia, thalamus, and brain stem1-5.Central effects of NMU, including the regulation of food intake, energy balance, stress response, and nociception, are mediated by NMUR2. In the brain, NMUR2 is predominant in the hypothalamic regions that are associated with regulation of food intake and energy balance5. NMUR2 has been suggested as a potential target for developing new therapeutics for the treatment of eating disorders, obesity, stress-related disorders, and pain5.Some studies have demonstrated that NMUR2 signaling in the paraventricular nucleus of the hypothalamus regulates consumption and preference for high-fat foods2.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Neuromedin-U receptor 2, NMU2 Receptor, G-protein coupled receptor TGR-1, G-protein coupled receptor FM-4, TGR1, GPR-FM4, SNORF72

Short Description :

A Rabbit Polyclonal Antibody to Neuromedin-U Receptor 2

Long Description :

Anti-NMUR2 (extracellular) Antibody (#ANR-072) is a highly specific antibody directed against an epitope of the rat Neuromedin-U receptor 2. The antibody can be used in western blot and live cell imaging applications. It has been designed to recognize NMUR2 from rat, human, and mouse samples.

Negative Control :

BLP-NR072

Positive Control :

Synonyms :

Neuromedin-U receptor 2, NMU2 Receptor, G-protein coupled receptor TGR-1, G-protein coupled receptor FM-4, TGR1, GPR-FM4, SNORF72

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NMUR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage