Anti-NMUR1 (extracellular) Antibody | Alomone Labs ANR-071 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NMUR1 (extracellular) Antibody

catalog :

ANR-071

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:500). 3-4. Anti-NMUR1 (extracellular) Antibody, preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).

image 2 :

Expression of NMUR1 in rat cerebellum. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1: 300), followed by goat anti-rabbit-AlexaFluor-488. A. NMUR1 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with NMUR1 (extracellular) Blocking Peptide (BLP-NR071), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-071

SKU :

ANR-071-CF_0.2 ml

Product Name :

Anti-NMUR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JJI5

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR071

Homology :

Human - 12 out of 16 amino acid residues identical; Mouse - 10 out of 16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DRLMWSMVSHWTDGLR, corresponding to amino acid residues 316 - 331 of rat NMUR1

Product Page - Scientific background :

Neuromedin U Receptor 1 (NMUR1) is one of the two receptors for the neuropeptide Neuromedin U (NMU). NMUR1 and the second receptor NMUR2 are members of the G protein coupled receptors (GPCRs) superfamily 1.NMU is a neuropeptide with potent activity on smooth muscles that is widely distributed in the gut and central nervous system. NMU interaction with one of its two receptors- NMU1R or NMU2R, mediates the stimulation of smooth muscles, increase of blood pressure, alteration of ion transport in the gut, control of local blood flow and regulation of adrenocortical function1-2.NMU1R is abundantly expressed in peripheral tissues including lung, GI tract, pancreatic islets, testis, adipose, immune tissues, and dorsal root ganglia2, and due to its diverse functions it is a very appealing drug and research candidate.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Neuromedin-U Receptor 1, G Protein-Coupled Receptor 66, GPR66

Short Description :

A Rabbit Polyclonal antibody to NMUR1 (extracellular)

Long Description :

Anti-NMUR1 (extracellular) Antibody (#ANR-071) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize NMUR1 from rat, mouse and human samples

Negative Control :

BLP-NR071

Positive Control :

Synonyms :

Neuromedin-U Receptor 1, G Protein-Coupled Receptor 66, GPR66

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NMUR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400-1:500

more info or order :

Alomone Labs product webpage