Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody | Alomone Labs ANR-063-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody

catalog :

ANR-063-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of Neuropilin-1 by direct flow cytometry in live intact mouse J774 macrophage cells: Cell surface detection of Neuropilin-1 by direct flow cytometry in live intact mouse J774 macrophage cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody (#ANR-063-F), (2.5µg).

product information

CAT :

ANR-063-F

SKU :

ANR-063-F_15 mcl

Product Name :

Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O14786

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR063

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)GGKHRENKVFMRK, corresponding to amino acid residues 502-514 of human neuropilin-1

Product Page - Scientific background :

Neuropilins (NRPs) are co-receptors for class 3 semaphorins, polypeptides with key roles in axonal guidance, and for members of the VEGF (vascular endothelial growth factor) family of angiogenic cytokines.Neuropilin 1 (NRP1) is a transmembrane glycoprotein and comprises a large extracellular region containing multiple domains, a single transmembrane domain and a small cytoplasmic domain, also with a number of protein interacting domains.Class 3 semaphorins bind NRP1 (and NRP2) with different, and partially overlapping, specificities and affinities. Plexin A1 and Plexin A2 are the major signaling receptors for class 3 semaphorins, and complex with NRPs and semaphorins to form a holoreceptor essential for mediating effects of semaphorins on growth-cone collapse and axonal guidance in neurogenesis. The major VEGF-A signaling receptor, VEGF receptor 2, forms complexes with NRP1 and is important for optimal VEGFR2 signaling and function in endothelial cells.Targeted disruption of the NRP1 gene has demonstrated an essential dual role of these molecules in neurogenesis and cardiovascular development. NRP1-null mice die with a spectrum of cardiovascular and neuronal defects1.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304

Short Description :

A Rabbit Polyclonal Antibody to Neuropilin-1 (NRP1) (extracellular) conjugated to the fluorescent dye FITC.

Long Description :

Anti-Neuropilin-1 (NRP1) (extracellular) Antibody (#ANR-063) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and live cell flow cytometry applications. It has been designed to recognize NRP1 from human, mouse, and rat samples. Anti-Neuropilin-1 (NRP1)-FITC Antibody (#ANR-063-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NRP1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (Unlabeled antibody, #ANR-063), and direct flow cytometry (Labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage