Anti-Neuropilin-2 (NRP2) (extracellular) Antibody | Alomone Labs ANR-062 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neuropilin-2 (NRP2) (extracellular) Antibody

catalog :

ANR-062

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4): - 1,2. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:200).3,4. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody, preincubated with Neuropilin-2/NRP2 (extracellular) Blocking Peptide (#BLP-NR062).

image 2 :

Expression of Neuropilin-2 in mouse substantia nigra pars compacta - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:400). A. NRP2 staining (green) reveals several neuronal profiles of NRP2 positive neurons that are distributed along the substantia nigra pars compacta. B. The same section stained for Calbindin-D28K (red) shows positive neurons along the substantia nigra pars compacta. C. Merge of the two images reveals co-localization in several neurons (arrows). DAPI counterstain is used to visualize nuclei of all cells (blue).

product information

CAT :

ANR-062

SKU :

ANR-062-CF_0.2 ml

Product Name :

Anti-Neuropilin-2 (NRP2) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O60462

Applications :

IF IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR062

Homology :

Rat, mouse - 11/12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)REDQGGEWKHGR, corresponding to amino acid residues 747-758 of human neuropilin-2

Product Page - Scientific background :

The neuropilins (NRPs) are single-pass multifunctional transmembrane glycoproteins that play an important role in cell development, immunity and cancer, and physiological and pathological angiogenesis. They were first discovered as regulators of nervous system development, acting as semaphorin co-receptors with plexins, as well as enhancers of cellular responses to members of the vascular endothelial growth factor (VEGF) family.The NRP family comprises two members: Neuropilin-1 (NRP1) and Neuropilin-2 (NRP2), which are usually expressed as homodimers but can also appear as heterodimers. NRP2 contains a large extracellular N-terminal which consists of five domains, a short transmembrane domain and a small cytoplasmic domain1-3. NRP2 is expressed in a wide variety of cells including endothelial cells, neurons, pancreatic islet cells, hepatocytes, melanocytes and osteoblasts. NRP2 has been shown to mediate proliferation, survival, and migration of tumor cells3.NRP2 knockout mice studies show that mice are viable without defects in blood vessels but display abnormal lymphatic development and defects of the central and peripheral nervous systems2.

Applications may also work in :

IF IFC IHC WB

Supplier :

Alomone Labs

Target :

Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2

Short Description :

A Rabbit Polyclonal Antibody to Neuropilin-2

Long Description :

Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize NRP2 from mouse, rat and human samples.

Negative Control :

BLP-NR062

Positive Control :

Synonyms :

Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NRP2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage