Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody | Alomone Labs ANR-062-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody

catalog :

ANR-062-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of Neuropilin-2 in live intact human THP-1 acute monocytic leukemia cell line: Cell surface detection of Neuropilin-2 in live intact human THP-1 acute monocytic leukemia cell line: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody (ANR-062-F), (2.5 µg).

product information

CAT :

ANR-062-F

SKU :

ANR-062-F_50 mcl

Product Name :

Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O60462

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR062

Homology :

Rat, mouse - 11/12 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)REDQGGEWKHGR, corresponding to amino acid residues 747-758 of human neuropilin-2

Product Page - Scientific background :

The neuropilins (NRPs) are single-pass multifunctional transmembrane glycoproteins that play an important role in cell development, immunity and cancer, and physiological and pathological angiogenesis. They were first discovered as regulators of nervous system development, acting as semaphorin co-receptors with plexins, as well as enhancers of cellular responses to members of the vascular endothelial growth factor (VEGF) family.The NRP family comprises two members: Neuropilin-1 (NRP1) and Neuropilin-2 (NRP2), which are usually expressed as homodimers but can also appear as heterodimers. NRP2 contains a large extracellular N-terminal which consists of five domains, a short transmembrane domain and a small cytoplasmic domain1-3. NRP2 is expressed in a wide variety of cells including endothelial cells, neurons, pancreatic islet cells, hepatocytes, melanocytes and osteoblasts. NRP2 has been shown to mediate proliferation, survival, and migration of tumor cells3.NRP2 knockout mice studies show that mice are viable without defects in blood vessels but display abnormal lymphatic development and defects of the central and peripheral nervous systems2.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2

Short Description :

A Rabbit Polyclonal Antibody to Neuropilin-2 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize NRP2 from mouse, rat, and human samples. Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody (#ANR-062-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NRP2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANR-062), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage