Anti-NEGR1 (extracellular) Antibody | Alomone Labs ANR-058 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NEGR1 (extracellular) Antibody

catalog :

ANR-058

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3 ) and rat brain synaptosomes (lanes 2 and 4 ) :

image 2 :

Western blot analysis of mouse pancreas lysate s (lanes 1 and 3) and mouse colon lysates (lanes 2 and 4):

image 3 :

Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4 ) , human Caco-2 colon adenocarcinoma cell line lysate (lanes 2 and 5 ) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 3 and 6 ) :

product information

CAT :

ANR-058

SKU :

ANR-058-CF_0.2 ml

Product Name :

Anti-NEGR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q80Z24

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR058

Homology :

Human, Rat – identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)HISPSAKPFENGQYLD, corresponding to amino acid residues 168 -183 of mouse NEGR1

Product Page - Scientific background :

Neuronal growth regulator 1 (NEGR1), also known as neurotractin or Kilon, is a member of the immunoglobulin superfamily’s IgLON subgroup. Structurally, IgLON proteins share three Ig-like C2-type domains and are anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor1,2. These proteins are implicated in neural development, supporting cellular interactions, neurite outgrowth, and synaptic formation3,4. NEGR1 is predominantly expressed in brain tissue, especially in the cerebral cortex, hippocampus, and hypothalamus. At the cellular level, it localizes to neuronal cell membranes where it regulates neurite extension and synapse stabilization1,5. NEGR1 activity is regulated through interaction with signaling molecules such as leukemia inhibitory factor receptor (LIFR), which affects downstream factors like lipocalin-2 (Lcn2) that are vital for neurogenesis and affective behaviors3. Functionally, NEGR1 modulates cell adhesion and neurite growth, processes essential for the proper development of neural circuits. It plays roles in both physiological and pathological states; for example, it is linked to hippocampal neurogenesis and synaptic plasticity, influencing behaviors related to anxiety and depression3,5. Dysfunctions in NEGR1 are associated with obesity, learning difficulties, psychiatric disorders, and mood dysregulation. Knockout studies in mice reveal that the loss of NEGR1 leads to reduced body mass, anxiety-like behaviors, and impaired neurogenesis2,4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Neuronal Growth Regulator 1, IgLON Family Member 4, IGLON4

Short Description :

A Rabbit polyclonal antibody to NEGR1 (extracellular).

Long Description :

Anti-NEGR1 (extracellular) Antibody (#ANR-058) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize NEGR1 from mouse, rat and human samples.

Negative Control :

BLP-NR058

Positive Control :

Synonyms :

Neuronal Growth Regulator 1, IgLON Family Member 4, IGLON4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NEGR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Product page antibodies part of immunoge ... :

(C)HISPSAKPFENGQYLD, corresponding to amino acid residues 168 -183 of mouse NEGR1

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage