Anti-Nectin-3/PVRL3 (extracellular) Antibody | Alomone Labs ANR-053 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nectin-3/PVRL3 (extracellular) Antibody

catalog :

ANR-053

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) testis membranes: - 1,2. Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (1:200).3,4. Anti-Nectin-3/PVRL3 (extracellular) Antibody, preincubated with Nectin-3/PVRL3 (extracellular) Blocking Peptide (#BLP-NR053).

image 2 :

Expression of Nectin-3 in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (1:100), followed by goat anti-rabbit-AlexaFluor-488. Nectin-3 immunoreactivity (green) appears in cells of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-053

SKU :

ANR-053-CF_0.2 ml

Product Name :

Anti-Nectin-3/PVRL3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JLB9

Applications :

IF IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR053

Homology :

Human, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ETITQISWEKIHGK, corresponding to amino acid residues 84-97 of mouse Nectin-3

Product Page - Scientific background :

Nectins are Ca2+-independent immunoglobulin-like cell adhesion molecules that localize at adherens junctions between two neighboring cells. They play an important role in mediating asymmetric homotypic and heterotypic cell-cell adhesions. The nectin family contains four members, nectin-1-4, which are encoded by the PVRL1-4 genes, respectively. Nectin-3 is localized mostly in dendrites and partly in axons and is crucial for the specific interactions between axons and dendrites and proper formation of the synapse1,2. Its structure consists of an extracellular region with three immunoglobulin-like loops, one V type and two C2 types.Nectins binds afadin at its c-terminal region and connects to the actin cytoskeleton. This interaction plays a major role in the formation of various cell-cell junctions in cooperation with or independently of cadherins cell adhesion molecules1,2.Nectins also regulates numerous cellular functions, such as cell movement, proliferation, polarization, survival and differentiation by interacting with various proteins1.

Applications may also work in :

IF IFC IHC WB

Supplier :

Alomone Labs

Target :

Nectin cell adhesion molecule 3, Poliovirus receptor-related 3, CD113

Short Description :

A Rabbit Polyclonal Antibody to Nectin-3/PVRL3

Long Description :

Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Nectin-3 from human, rat, and mouse samples.

Negative Control :

BLP-NR053

Positive Control :

Synonyms :

Nectin cell adhesion molecule 3, Poliovirus receptor-related 3, CD113

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NECTIN3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage