Anti-Nectin-2/PVRL2 (extracellular) Antibody | Alomone Labs ANR-052 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nectin-2/PVRL2 (extracellular) Antibody

catalog :

ANR-052

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Oliveras A, Serrano Novillo C, Moreno C, de la Cruz A, Valenzuela C, Soeller C, et al. The unconventional biogenesis of Kv7.1-KCNE1 complexes. Sci Adv. 2020;6:eaay4472 pubmed publisher

image

image 1 :

Western blot analysis of rat spleen lysate (lanes 1 and 4), human Jurkat T cell leukemia cell lysate (lanes 2 and 5) and human K562 erythroleukemia cell lysate (lanes 3 and 6): - 1-3. Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052), (1:200).4-6. Anti-Nectin-2/PVRL2 (extracellular) Antibody, preincubated with Nectin-2/PVRL2 (extracellular) Blocking Peptide (#BLP-NR052).

image 2 :

Expression of Nectin-2 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052), (1:600), followed by goat-anti-rabbit-AleaxaFluor-488. Nectin-2 staining (green) appears in in apical dendrites of CA1 pyramidal neurons (vertical arrows) and in astrocytes (horizontal arrows). Cell nuclei are stained with DAPI (blue).

image 3 :

Expression of Nectin-2 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Nectin-2/PVRL2 (extracellular) Antibody(#ANR-052) (1:600) followed by goat-anti-rabbit-AleaxaFluor-488. Nectin-2 staining (green) appears in in apical dendrites of CA1 pyramidal neurons (vertical arrows) and in astrocytes (horizontal arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-052

SKU :

ANR-052-CF_0.2 ml

Product Name :

Anti-Nectin-2/PVRL2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P32507

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR052

Homology :

Rat - identical; human - 10/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RVIAQPENHAEAQE, corresponding to amino acid residues 148-161 of mouse Nectin-2

Product Page - Scientific background :

The Nectin family is known as cell-cell adhesion molecules and comprises four members in this family: nectin-1, nectin-2, nectin-3 and nectin-4 which are encoded by PVRL1, PVRL2, PVRL3, and PVRL4 genes respectively1. They are Ca2+-independent immunoglobulin-like CAMs and are able to trans-interact1.They are localized at adherens junctions between two neighboring cells and binds to afadin through their C-terminal region1.Nectins, apart from a secreted nectin-1γ, exhibit three immunoglobulin-like loops (one V type and two C2 type) in their extracellular region, a single trans membrane segment and a cytoplasmic tail. Nectins first form a cis-homo-dimer which later on undergoes lateral cluster formation on the cell surface1. Nectins regulate a variety of cellular functions in addition to cell-cell junction formation such as cell polarity, movement, proliferation, differentiation, and survival1.The PVRL2 gene (which encodes nectin-2) encodes two mRNA isoforms of 3 kb and 4.4 kb both widely expressed in various human tissues including hematopoietic cells of the myelo-monocytic and megakaryocytic lineages and detected also on the surface of endothelial cells2.Nectin-2 acts as mediator of herpes simplex virus invasion into host cell. A single nucleotide polymorphism in PVRL2 shows an association with later onset of Alzheimers Disease3.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Poliovirus receptor-related protein 2, Herpes virus entry mediator B, PRR2, PVRR2, CD112

Short Description :

A Rabbit Polyclonal Antibody to Nectin-2/PVRL2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Poliovirus receptor-related protein 2. Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052) can be used in western blot, immunohistochemistry, and flow cytometry applications. It has been designed to recognize Nectin-2 from mouse, rat and human samples.

Negative Control :

BLP-NR052

Positive Control :

Synonyms :

Poliovirus receptor-related protein 2, Herpes virus entry mediator B, PRR2, PVRR2, CD112

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NECTIN2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage