Anti-Nectin-1/PVRL1 (extracellular) Antibody | Alomone Labs ANR-051 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nectin-1/PVRL1 (extracellular) Antibody

catalog :

ANR-051

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:200).3,4. Anti-Nectin-1/PVRL1 (extracellular) Antibody, preincubated with Nectin-1/PVRL1 (extracellular) Blocking Peptide (#BLP-NR051).

image 2 :

Expression of Nectin-1 in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:100). A. Nectin-1 staining (red) is apparent in Purkinje neurons and their dendritic tree (arrow). B. Cell nuclei in the same section are visualized with DAPI (blue). C. Merge of the two images.

image 3 :

Expression of Nectin-1 in rat PC12 cells - Cell surface detection ofNectin-1 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells withAnti-Nectin-1/PVRL1 (extracellular) Antibody(#ANR-051) (1:100) followed by goat anti-rabbit-AlexaFluor-594secondary antibody (red). B. Live image of the cells. C. Merge of the two images.

product information

CAT :

ANR-051

SKU :

ANR-051-CF_0.2 ml

Product Name :

Anti-Nectin-1/PVRL1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q15223

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR051

Homology :

Rat - identical; mouse - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)GKPPSVVSWETRLK, corresponding to amino acid residues 177-190 of human Nectin-1

Product Page - Scientific background :

Nectins, which were originally identified as virus receptors, are members of the cell-cell adhesion molecule (CAM) family. They are Ca2+-independent immunoglobulin-like CAMs. The nectin family comprises four members, nectin-1, nectin-2, nectin-3 and nectin-4, which are encoded by the PVRL1, PVRL2, PVRL3 and PVRL4 genes, respectively1. In the central nervous system, these cell adhesion molecules aggregate in formations, termed puncta adherentia junctions, which are mechanical adhesive sites that connect pre- and postsynaptic membranes2.All nectins contain an extracellular region with three immunoglobulin-like loops (one V type and two C2 types), a single membrane-spanning region and a cytoplasmic tail. Nectins directly bind afadin, an F-actin-binding protein, through their cytoplasmic tails1.Nectin-1 is expressed at cell-cell junctions in human and mouse epidermis. In mice lacking the gene encoding nectin-1 (Pvrl1−/− mice), the expression of loricrin, a differentiation marker and a major component of cornified cell envelopes in the epidermis, is downregulated and newborn pups have a shiny and slightly reddish skin3. Mutations in human PVRL1 are implicated in cleft lip or palate-ectodermal dysplasia syndromes, which includes Zlotogora-Ogur syndrome and Margarita Island ectodermal dysplasia4. Both of these are autosomal recessive disorders that are clinically characterized by unusual facial appearance, dental anomalies, hypotrichosis, palmoplantar hyperkeratosis and onychodysplasia, syndactyly, cleft lip or palate, and in some cases, mental retardation5.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Poliovirus receptor-related protein 1, Herpes virus entry mediator C, PRR1, CD111

Short Description :

A Rabbit Polyclonal Antibody to Nectin-1/PVRL1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of human Poliovirus receptor-related protein 1. Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051) can be used in western blot, immunocytochemistry and indirect flow cytometry applications. It is specially suited to detect Nectin-1 in live cells. It has been designed to recognize Nectin-1 from rat, mouse and human samples and will recognize all known isoforms of Nectin-1, including the secreted form (also known as isoform gamma).

Negative Control :

BLP-NR051

Positive Control :

Synonyms :

Poliovirus receptor-related protein 1, Herpes virus entry mediator C, PRR1, CD111

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NECTIN1

AB Specifictiy :

This antibody will recognize all known isoforms of Nectin-1, including the secreted form (also known as isoform gamma).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage