antibody

Anti-Nectin-1/PVRL1 (extracellular)-FITC Antibody

ANR-051-F

polyclonal

domestic rabbit

FITC

NA

human, mouse, rat

ANR-051-F_15 mcl

Anti-Nectin-1/PVRL1 (extracellular)-FITC Antibody

Antibodies

Antibodies

Polyclonal

Q15223

FC LCI

Human Rat Mouse

Rabbit

BLP-NR051

Rat - identical; mouse - 13 out of 14 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Extracellular, N-terminus

Fluorescein isothiocyanate (FITC).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

(C)GKPPSVVSWETRLK, corresponding to amino acid residues 177-190 of human Nectin-1

Nectins, which were originally identified as virus receptors, are members of the cell-cell adhesion molecule (CAM) family. They are Ca2+-independent immunoglobulin-like CAMs. The nectin family comprises four members, nectin-1, nectin-2, nectin-3 and nectin-4, which are encoded by the PVRL1, PVRL2, PVRL3 and PVRL4 genes, respectively1. In the central nervous system, these cell adhesion molecules aggregate in formations, termed puncta adherentia junctions, which are mechanical adhesive sites that connect pre- and postsynaptic membranes2.All nectins contain an extracellular region with three immunoglobulin-like loops (one V type and two C2 types), a single membrane-spanning region and a cytoplasmic tail. Nectins directly bind afadin, an F-actin-binding protein, through their cytoplasmic tails1.Nectin-1 is expressed at cell-cell junctions in human and mouse epidermis. In mice lacking the gene encoding nectin-1 (Pvrl1−/− mice), the expression of loricrin, a differentiation marker and a major component of cornified cell envelopes in the epidermis, is downregulated and newborn pups have a shiny and slightly reddish skin3. Mutations in human PVRL1 are implicated in cleft lip or palate-ectodermal dysplasia syndromes, which includes Zlotogora-Ogur syndrome and Margarita Island ectodermal dysplasia4. Both of these are autosomal recessive disorders that are clinically characterized by unusual facial appearance, dental anomalies, hypotrichosis, palmoplantar hyperkeratosis and onychodysplasia, syndactyly, cleft lip or palate, and in some cases, mental retardation5.

FC LCI

Alomone Labs

Poliovirus receptor-related protein 1, Herpes virus entry mediator C, PRR1, CD111

A Rabbit Polyclonal Antibody to TRPM2 (extracellular) conjugated to the fluorescent dye FITC

Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize Nectin-1 from human, mouse and rat samples. It will recognize all known isoforms of Nectin-1, including the secreted form (also known as isoform gamma). Anti-Nectin-1/PVRL1 (extracellular)-FITC Antibody (#ANR-051-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

RIC-001-F

NA

Poliovirus receptor-related protein 1, Herpes virus entry mediator C, PRR1, CD111

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

NECTIN1

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #ANR-051), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information