Anti-DSCAM (extracellular) Antibody | Alomone Labs ANR-046 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-DSCAM (extracellular) Antibody

catalog :

ANR-046

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain synaptosomes lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):

image 2 :

Western blot analysis of human HepG2 hepatocellular carcinoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4): .

image 3 :

Expression of DSCAM in rat cerebellum.

product information

CAT :

ANR-046

SKU :

ANR-046-CF_0.2 ml

Product Name :

Anti-DSCAM (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9ERC8

Applications :

IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR046

Homology :

Rat - identical, Human - 15 out of 17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RITGLNHANLIMDHMVK, corresponding to amino acid residues 361 - 377 of mouse DSCAM

Product Page - Scientific background :

Down syndrome cell adhesion molecule, DSCAM, also known as CHD2, is a member of the immunoglobulin superfamily of cell adhesion molecules (Ig-CAMs). DSCAM is classified as a type I membrane protein with an extracellular domain consisting of ten immunoglobulin (Ig) and six fibronectin type III (FNIII) repeats and an intracellular domain that does not contain any identifiable motifs.1The gene coding for the DSCAM protein is located on the human chromosome 21, specifically in the so-called Down syndrome critical region (DSCR), the trisomy of which is considered a determinant for the intellectual disability associated with Down's syndrome. Therefore, the potential involvement of DSCAM in the developing neural system, has received considerable attention.2,3DSCAM is widely expressed in the vertebrate central nervous system where it plays a crucial role in neural development. DSCAM was found to have a critical role for the development of neural circuits, playing a role in processes such as axon guidance, dendritic arborization, and synapse formation. 2-5DSCAM is involved in axon guidance, a process by which developing neurons extend their axons to establish specific connections with target cells. DSCAM serves as a receptor for Netrin-1, a member of a conserved family of secreted proteins that promote axon outgrowth and guide growth cone navigation.2,6The molecular mechanisms by which DSCAM contributes to autism spectrum disorders (ASDs), is not clear, but it may involve altered synaptic transmission, probably through its interaction with other adhesion molecules such as neuroligins (NLGNs) and neurexin1 (NRXN1). In fact, deletion of DSCAM in neurons results in abnormal spine maturation, altered synaptic transmission, as well as autism-like behaviors.5

Applications may also work in :

IHC LCI WB

Supplier :

Alomone Labs

Target :

Down Syndrome Cell Adhesion Molecule, DS Cell Adhesion Molecule, CHD2

Short Description :

A Rabbit Polyclonal antibody to L1CAM (extracellular)

Long Description :

Anti-DSCAM (extracellular) Antibody (#ANR-046) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DSCAM from mouse, rat and human samples.

Negative Control :

BLP-NR046

Positive Control :

Synonyms :

Down Syndrome Cell Adhesion Molecule, DS Cell Adhesion Molecule, CHD2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DSCAM

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage