Anti-NrCAM (extracellular)-FITC Antibody | Alomone Labs ANR-045-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NrCAM (extracellular)-FITC Antibody

catalog :

ANR-045-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of NrCAM by direct flow cytometry in live intact mouse P815 mastocytoma cells:

image 2 :

Cell surface detection of NrCAM by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:

product information

CAT :

ANR-045-F

SKU :

ANR-045-F_50 mcl

Product Name :

Anti-NrCAM (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97686

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR045

Homology :

Mouse - 15/16 amino acid residues identical; human - 13/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KDNGELPN DERFSVD, corresponding to amino acid residues 583-597 of rat NrCAM

Product Page - Scientific background :

NrCAM (Neuron-glia related cell adhesion molecule) is a cell adhesion molecule that belongs to the immunoglobulin-class (Ig) of the L1 family. NrCAM promotes axon growth and repulsion through trans homophilic binding of extracellular domains and intracellular coupling of the conserved cytoplasmic domains to the actin cytoskeleton. This interaction occurs through ankyrin B and ezrin-radixin-moesin adaptors. In addition, NrCAM is responsible for controlling dendritic spine densities, axonal guidance and targeting, and neurite outgrowth by acting as a co-receptor molecule at the neuronal cell surface1,2.NrCAM is highly expressed in the cortex1,3. Its structure contains a PDZ binding motif with the potential to bind PSD scaffold proteins PSD-95 and SAP102. The Ig domains of the protein mediate homophilic and heterophilic binding to multiple ligands2,3.Genetic polymorphisms in the NrCAM gene are linked to autism spectrum disorders, intellectual disability and addictive behavior. NrCAM knockout mice demonstrate autism spectrum disorder related behaviors including impaired sociability, cognitive inflexibility, hypersensitivity to sensory stimuli and altered visual cortical responses.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo

Short Description :

A Rabbit Polyclonal Antibody to NrCAM (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-NrCAM (extracellular) Antibody (#ANR-045) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize NrCAM from mouse, rat and human samples. Anti-NrCAM (extracellular)-FITC Antibody (#ANR-045-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize NrCAM from mouse, rat and human samples.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NRCAM

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANR-045), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage