Anti-NCAM2 (extracellular) Antibody | Alomone Labs ANR-042 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NCAM2 (extracellular) Antibody

catalog :

ANR-042

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody (#ANR-042), (1:200).3,4. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody, preincubated with NCAM2 (extracellular) Blocking Peptide (#BLP-NR042).

image 2 :

Expression of NCAM2 in mouse brain - Immunohistochemical staining of mouse free-floating frozen brain sections using Anti-NCAM2 (extracellular) Antibody (#ANR-042). A. In the hippocampal CA3 region, NCAM2 immunoreactivity (green) appears in the pyramidal layer (P, arrows). B. In the olfactory bulb, NCAM2 staining (green) appears in the Mitral cell layer (MCL, arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).

product information

CAT :

ANR-042

SKU :

ANR-042-CF_0.2 ml

Product Name :

Anti-NCAM2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35136

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR042

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KEDQWLEKKVQGNKDH, corresponding to amino acid residues 635-650 of mouse NCAM-2

Product Page - Scientific background :

Neural cell adhesion molecule 2 (NCAM-2), a protein also known as olfactory cell adhesion molecule (OCAM), belongs to the immunoglobulin superfamily of cell adhesion molecules that participate in homophilic trans-interactions. NCAM-2 is highly expressed in the nervous system and participates in numerous cellular events, such as survival, migration, and differentiation, neurite outgrowth, and formation and plasticity of synapses1,2. The NCAM-2 structure consists of five immunoglobulin (Ig) domains followed by two fibronectin-type 3 (Fn3) domains and eight potential sites for N-linked glycosylation3.Overexpression of NCAM-2 is believed to be a contributing factor to Down syndrome. There is also a genetic association between NCAM-2 and Alzheimer's disease, where NCAM-2 is involved in the pathogenic pathway to the senile plaques that concentrate in the Alzheimer's disease brain1.During human embryonic development, NCAM-2 is expressed in several tissues, including lung, liver, and kidney with the highest expression in the brain. NCAM-2 expression level rises around postnatal day 21 and remains high during adulthood. Studies conducted on NCAM-2 deficient mice show that the protein is important for the development of the brain and the olfactory system1.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Neural cell adhesion molecule 2, NCAM-2, R4B12, Ocam, Rncam, NCAM21, Neural cell adhesion molecule RB-8

Short Description :

A Rabbit Polyclonal Antibody to Neural Cell Adhesion Molecule 2

Long Description :

Anti-NCAM2 (extracellular) Antibody (#ANR-042) is a highly specific antibody directed against mouse Neural cell adhesion molecule 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NCAM2 from rat, mouse, and human samples.

Negative Control :

BLP-NR042

Positive Control :

Synonyms :

Neural cell adhesion molecule 2, NCAM-2, R4B12, Ocam, Rncam, NCAM21, Neural cell adhesion molecule RB-8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NCAM2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage