Anti-CD56/NCAM1 (extracellular) Antibody | Alomone Labs ANR-041 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CD56/NCAM1 (extracellular) Antibody

catalog :

ANR-041

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, western blot knockout validation

more info or order :

Alomone Labs product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:400; loading ...; fig 5a, s3	Delgado C, Bu L, Zhang J, Liu F, Sall J, Liang F, et al. Neural cell adhesion molecule is required for ventricular conduction system development. Development. 2021;148: pubmed publisher
	Voskobiynyk Y, Battu G, Felker S, Cochran J, Newton M, Lambert L, et al. Aberrant regulation of a poison exon caused by a non-coding variant in a mouse model of Scn1a-associated epileptic encephalopathy. PLoS Genet. 2021;17:e1009195 pubmed publisher
	Huang R, Yuan D, Li S, Liang X, Gao Y, Lan X, et al. NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis. J Cell Biol. 2020;219: pubmed publisher

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 6), rat cerebellum (lanes 2 and 7), rat eye (lanes 3 and 8), human SH-SY5Y brain neuroblastoma (lanes 4 and 9) and PC12 rat pheochromocytoma (lanes 5 and 10) cells lysates: - 1-5. Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041), (lanes 1-3 1:400 and lanes 4-5 1:200).6-10. Anti-CD56/NCAM1 (extracellular) Antibody, preincubated with CD56/NCAM1 (extracellular) Blocking Peptide (#BLP-NR041).

image 2 :

Expression of NCAM1 in rat olfactory bulb - Immunohistochemical staining of rat olfactory bulb frozen section using Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041), (1:300). A. NCAM1 staining (green) appears in glomeruli (G) and is particularly intense in the outer border (arrows). B. Nuclei are stained using DAPI as the counterstain. C. Merged image of A and B panels.

image 3 :

Expression of NCAM1 in rat PC12 cells - Cell surface detection ofNCAM1 in intact living rat pheochromocytoma (PC12) cells usingAnti-CD56 (NCAM1) (extracellular) Antibody(#ANR-041) (1:100).A B. Extracellular staining of cells merged with a live view of the cells.

product information

CAT :

ANR-041

SKU :

ANR-041-CF_0.2 ml

Product Name :

Anti-CD56/NCAM1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P13596

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR041

Homology :

Mouse - identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat NCAM-1

Product Page - Scientific background :

Neural cell adhesion molecule 1 (NCAM-1) is a member of a large family of cell-surface glycoproteins and plays a major role during development and controls various functions in the nervous system such as cell migration, neurite growth, axonal guidance, and synaptic plasticity1. NCAM-1 mediates adhesion between cells through homophilic bonds formed between its extracellular domains, which comprise five tandem Ig domains and two juxtamembrane fibronectin type III (Fn III) domains2. There are three major protein isoforms of NCAM-1: 180 kd, 140 kd, and 120 kd. The 180-kd and 140-kd isoforms of NCAM-1 are transmembrane proteins, whereas the 120-kd isoform is linked to the plasma membrane via a glycosyl phosphatidyl-inositol (GPI) lipid anchor3. NCAM-1 is widely expressed in the central nervous system, skeletal muscle and natural killer cells4.Several lines of evidence have supported the idea that dysregulation of NCAM-1 isoforms in the brain might be involved in the pathophysiology of neuropsychiatric disorders, particularly bipolar affective disorder5.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Neural cell adhesion molecule 1, NCAM-1

Short Description :

A Rabbit Polyclonal Antibody to Neural Cell Adhesion Molecule 1 (CD56)

Long Description :

Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041) is a highly specific antibody directed against rat Neural cell adhesion molecule 1. The antibody can be used in western blot, live cell imaging, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize NCAM1 from rat, mouse and human samples.

Negative Control :

BLP-NR041

Positive Control :

Synonyms :

Neural cell adhesion molecule 1, NCAM-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NCAM1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage