Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs ANR-041-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody

catalog :

ANR-041-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Delgado C, Bu L, Zhang J, Liu F, Sall J, Liang F, et al. Neural cell adhesion molecule is required for ventricular conduction system development. Development. 2021;148: pubmed publisher

image

image 1 :

Expression of CD56 in rat brain - Immunohistochemical staining of rat olfactory bulb free-floating frozen sections using Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody (#ANR-041-AG), (1:60). CD56 immunoreactivity (green) appears in glomerular structures (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).

product information

CAT :

ANR-041-AG

SKU :

ANR-041-AG_50 mcl

Product Name :

Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P13596

Applications :

IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR041

Homology :

Mouse - identical; human - 13/15 amino acids residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat NCAM-1

Product Page - Scientific background :

Neural cell adhesion molecule 1 (NCAM-1) is a member of a large family of cell-surface glycoproteins and plays a major role during development and controls various functions in the nervous system such as cell migration, neurite growth, axonal guidance, and synaptic plasticity1. NCAM-1 mediates adhesion between cells through homophilic bonds formed between its extracellular domains, which comprise five tandem Ig domains and two juxtamembrane fibronectin type III (Fn III) domains2. There are three major protein isoforms of NCAM-1: 180 kd, 140 kd, and 120 kd. The 180-kd and 140-kd isoforms of NCAM-1 are transmembrane proteins, whereas the 120-kd isoform is linked to the plasma membrane via a glycosyl phosphatidyl-inositol (GPI) lipid anchor3. NCAM-1 is widely expressed in the central nervous system, skeletal muscle and natural killer cells4.Several lines of evidence have supported the idea that dysregulation of NCAM-1 isoforms in the brain might be involved in the pathophysiology of neuropsychiatric disorders, particularly bipolar affective disorder5.

Applications may also work in :

IHC

Supplier :

Alomone Labs

Target :

NCAM-1, CD56

Short Description :

A Rabbit Polyclonal Antibody to CD56/NCAM1

Long Description :

Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging, and immunohistochemistry applications. It has been designed to recognize CD56 from rat, mouse and human samples. Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody (#ANR-041-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody is suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

NCAM-1, CD56

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NCAM1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANR-041), and immunohistochemical staining (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage