Anti-Neuroligin 2 (extracellular) Antibody | Alomone Labs ANR-036 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neuroligin 2 (extracellular) Antibody

catalog :

ANR-036

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; rat; loading ...; fig 1c immunocytochemistry; rat; 1:100; loading ...; fig 3a	Halff E, Szulc B, Lesept F, Kittler J. SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Cell Rep. 2019;29:2599-2607.e6 pubmed publisher
	Bohnsack J, Carlson S, Morrow A. Differential regulation of synaptic and extrasynaptic Î±4 GABA(A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons. Neuropharmacology. 2016;105:124-132 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and rat PC12 pheochromocytoma cells (lanes 3 and 6): - 1-3. Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036), (1:400).4-6. Anti-Neuroligin 2 (extracellular) Antibody, preincubated with Neuroligin 2 (extracellular) Blocking Peptide (#BLP-NR036).

image 2 :

Expression of NLGN2 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. NLGN2 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.

product information

CAT :

ANR-036

SKU :

ANR-036-CF_0.2 ml

Product Name :

Anti-Neuroligin 2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62888

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR036

Homology :

Mouse - identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DLGPRAYDRFPGDS, corresponding to amino acid residues 657-670 of rat NLGN2

Product Page - Scientific background :

Neuroligins (NLGNs) are a family of postsynaptic cell adhesion molecules that play an important role in synaptic development and function1. Four genes encode for the different members of the neuroligin family in mammals: NLGN 1-4, which are differentially enriched in postsynaptic specializations of synapses2. Neuroligin 2 (NLGN2) is expressed in neurons in the brain and also in pancreatic β cells where it facilitates insulin secretion3.Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners3. NLGN2 plays a role in synapse function and synaptic signal transmission, especially via γ-aminobutyric acid receptors (GABA(A) receptors). NLGN2 modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing4.Recent studied identified a loss-of-function mutation of NLGN2 in schizophrenia patients, suggesting an indispensable role of NLGN2 in regulating GABAergic functions5.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

NLGN2, NL2

Short Description :

A Rabbit Polyclonal Antibody to Neuroligin 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Neuroligin 2. Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NLGN2 from rat, mouse and human samples.

Negative Control :

BLP-NR036

Positive Control :

Synonyms :

NLGN2, NL2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NLGN2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage