Anti-Neuroligin 1 (extracellular) Antibody | Alomone Labs ANR-035 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neuroligin 1 (extracellular) Antibody

catalog :

ANR-035

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Ohta K, Suzuki S, Warita K, Sumitani K, Tenkumo C, Ozawa T, et al. The effects of early life stress on the excitatory/inhibitory balance of the medial prefrontal cortex. Behav Brain Res. 2020;379:112306 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035), (1:800).3,4. Anti-Neuroligin 1 (extracellular) Antibody preincubated with Neuroligin 1 (extracellular) Blocking Peptide (#BLP-NR035).

image 2 :

Expression of NLGN1 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035) followed by anti-rabbit-AlexaFluor-594 secondary antibody. A. NLGN1 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.

image 3 :

Expression of NLGN1 in rat hippocampus and cortex - Immunohistochemical staining of free floating frozen section of rat hippocampal CA1 region (A B) and rat parietal cortex (C D) usingAnti-Neuroligin 1 (extracellular)Antibody (#ANR-035) (1:300). A. NLGN1 staining (green) appears in several neuronal profiles in the pyramidal layer (arrows). C. NLGN1 staining (green) is detected in several neuronal profiles including pyramidal cells and their apical dendrite (arrows). B D. Nuclei are stained using DAPI as the counterstain (blue).

product information

CAT :

ANR-035

SKU :

ANR-035-CF_0.2 ml

Product Name :

Anti-Neuroligin 1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62765

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR035

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KVPSTDITLRPTRK, corresponding to amino acid residues 648-661 of rat NLGN1

Product Page - Scientific background :

The NLGN1 gene encodes a member of a family of neuronal cell surface proteins. Neuroligins act as ligands for β-neurexins, cell adhesion proteins located presynaptically. Neuroligin and β-neurexin "shake hands," resulting in the connection between the two neurons and the production of a synapse. Neuroligin 1 may be involved in the formation and remodeling of central nervous system synapses1.Proteins of this family may also mediate signaling by recruiting and stabilizing key synaptic components. This is done by interacting with other postsynaptic proteins to localize neurotransmitter receptors and channels in the postsynaptic density as the cell matures1.Mutations in the neuroligin genes are implicated in autism and other cognitive disorders2.Neuroligins have been found to play a role in angiogenesis, specifically in human pheripheral tissues3.Neuroligins bind with the help of Ca2+ to the α-neurexin LNS (laminin, neurexin and sex hormone-binding globulin-like folding units) domains and to the β-neurexin LNS domain which then establishes a heterophilic trans-synaptic recognition code4. This binding results in a heterotetramer made up of a neuroligin-1 dimer and two neurexin-1 β monomers5.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

NLGN1, NL1

Short Description :

A Rabbit Polyclonal Antibody to Neuroligin 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Neuroligin 1. Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NLGN1 from rat, mouse and human samples.

Negative Control :

BLP-NR035

Positive Control :

Synonyms :

NLGN1, NL1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NLGN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage