Anti-Neurexin 3α (extracellular) Antibody | Alomone Labs ANR-033 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neurexin 3α (extracellular) Antibody

catalog :

ANR-033

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Delgado C, Bu L, Zhang J, Liu F, Sall J, Liang F, et al. Neural cell adhesion molecule is required for ventricular conduction system development. Development. 2021;148: pubmed publisher

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 5), rat brain (lanes 2 and 6), human brain neuroblastoma (SH-SY5Y) cells (lanes 3 and 7) and rat Pheochromocytoma (PC-12) cells (lanes 4 and 8): - 1-4. Anti-Neurexin 3α (extracellular) Antibody (#ANR-033), (1:200).5-8. Anti-Neurexin 3α (extracellular) Antibody, preincubated with Neurexin 3α (extracellular) Blocking Peptide (#BLP-NR033).

image 2 :

Expression of Neurexin 3α in rat and mouse brain - Immunohistochemical staining of rat entorhinal cortex (A) and mouse striatum and lateral septum (B) using Anti-Neurexin 3α (extracellular) Antibody (#ANR-033), (1:400). A. Nrxn3α staining (green) appears in neuronal outlines in entorhinal cortex (arrow). B. Nrxn3α staining appeared in axonal processes in striatum (horizontal arrow) and in neuronal cell bodies in lateral septum (vertical arrows). DAPI is used as the counterstain (blue).

image 3 :

Expression ofNeurexin 3?in rat and mouse brain - Immunohistochemical staining of rat entorhinal cortex (A) and mouse striatum and lateral septum (B) usingAnti-Neurexin 3? (extracellular) Antibody (#ANR-033) (1:400). A. Nrxn3? staining (green) appears in neuronal outlines in entorhinal cortex (arrow). B. Nrxn3? staining appeared in axonal processes in striatum (horizontal arrow) and in neuronal cell bodies in lateral septum (vertical arrows).DAPI is used as the counterstain (blue).

product information

CAT :

ANR-033

SKU :

ANR-033-CF_0.2 ml

Product Name :

Anti-Neurexin 3α (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q07310

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR033

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RYLRWDASTRSDLS, corresponding to amino acid residues 39-52 of rat Nrxn3α

Product Page - Scientific background :

Neurexins (NRXNs) are a family of transmembrane, synaptic adhesion molecules. NRXNS were identified as receptors for α-latrotoxin, a presynaptic toxin that triggers massive neurotransmitter release1. Neurexins are largely presynaptic proteins that form a trans-synaptic cell-adhesion complex with postsynaptic neuroligins2. They are encoded by three genes (NRXN1, NRXN2 and NRXN3), each using an upstream promoter to produce the longer α-isoform (α-NRXNs) and a downstream promoter to generate a shorter β-isoform (β-NRXNs).The α-isoforms and β-isoforms of each neurexin are single-pass transmembrane proteins maintaining identical transmembrane and intracellular domains but having distinct extracellular domains. NRXNs in neurons localize to the presynaptic membrane and bind trans-synaptically to postsynaptic adhesion molecules and receptors3. Neurexins are expressed in all neurons, and are subject to extensive alternative splicing, generating >1,000 splice variants, some of which exhibit highly regulated developmental and spatial expression patterns4.The structure of α-Neurexins is composed of 6 LNS domains (laminin, neurexin, sex-hormone-binding protein domain) and 3 EGF-like domains. In the mammalian brain they are known to interact with neuroligins, dystroglycan and neurexophilins. Neurexin 3α splicing can give rise to secreted and transmembrane forms of the protein. The biological function of the secreted form remains unknown5.Changes in Neurexin 3α are associated with autism, drug addiction and obesity6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Nrxn3, NRX3A, Neurexin III-alpha

Short Description :

A Rabbit Polyclonal Antibody to Neurexin 3α

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Neurexin 3α. Anti-Neurexin 3α (extracellular) Antibody (#ANR-033) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is therefore, highly suited to recognize Nrxn3α in living cells. It has been designed to recognize Nrxn3α from rat, mouse and human samples.

Negative Control :

BLP-NR033

Positive Control :

Synonyms :

Nrxn3, NRX3A, Neurexin III-alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NRXN3

AB Specifictiy :

Designed to recognize Neurexin 3α and not Neurexin 3β.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage